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1. (WO2014059548) METHODS AND COMPOSITIONS FOR ASSESSING SPERMATOZOA IN A SEMEN SAMPLE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2014/059548 International Application No.: PCT/CA2013/050788
Publication Date: 24.04.2014 International Filing Date: 17.10.2013
IPC:
C12Q 1/68 (2006.01) ,C12Q 1/06 (2006.01) ,G01N 1/00 (2006.01) ,G01N 15/10 (2006.01) ,G01N 33/483 (2006.01) ,G01N 33/567 (2006.01) ,C12N 5/076 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
06
Quantitative determination
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15
Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10
Investigating individual particles
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
483
Physical analysis of biological material
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
566
using specific carrier or receptor proteins as ligand binding reagent
567
utilising isolate of tissue or organ as binding agent
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
076
Sperm cells; Spermatogonia
Applicants:
BOILARD, Mathieu [CA/CA]; CA
MASSICOTTE, Lyne [CA/CA]; CA
Inventors:
BOILARD, Mathieu; CA
MASSICOTTE, Lyne; CA
Agent:
SMART & BIGGAR; 1000 De La Gauchetière Street West Suite 3300 Montreal, Québec H3B 4W5, CA
Priority Data:
61/715,36218.10.2012US
Title (EN) METHODS AND COMPOSITIONS FOR ASSESSING SPERMATOZOA IN A SEMEN SAMPLE
(FR) PROCÉDÉS ET COMPOSITIONS POUR ÉVALUER DES SPERMATOZOÏDES DANS UN ÉCHANTILLON DE SPERME
Abstract:
(EN) The present invention relates to the field of mammalian reproduction and provides methods, compositions and kits for detecting and assessing spermatozoa and intervening cells in a semen sample which are applicable to human and veterinary uses. Various aspects of the present invention provide for a cytometric multiparametric approach for determining spermatozoa concentration in a semen sample, wherein the cytometric multiparametric approach involves use of one or more spermatozoa-specific detection agents for detection of spermatozoa in the semen sample and one or more intervening cells-specific detection agents.
(FR) La présente invention concerne le domaine de la reproduction des mammifères et concerne des procédés, des compositions et des trousses de détection et d'évaluation des spermatozoïdes et des cellules intermédiaires dans un échantillon de sperme qui peuvent être appliqués à des utilisations humaines et vétérinaires. Divers aspects de la présente invention concernent une approche multiparamétrique cytométrique pour déterminer la concentration en spermatozoïdes dans un échantillon de sperme, l'approche multiparamétrique cytométrique mettant en jeu l'utilisation d'un ou plusieurs agents de détection spécifiques de spermatozoïdes pour la détection des spermatozoïdes dans l'échantillon de sperme, et un ou plusieurs agents de détection spécifiques de cellules intermédiaires.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)