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1. (WO2014037460) IMMOBILISED CYCLIN-DEPENDENT KINASE 4 FUSION PROTEINS AND USES THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2014/037460 International Application No.: PCT/EP2013/068399
Publication Date: 13.03.2014 International Filing Date: 05.09.2013
IPC:
C07K 14/47 (2006.01) ,C12N 9/12 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
46
from vertebrates
47
from mammals
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10
Transferases (2.)
12
transferring phosphorus containing groups, e.g. kinases (2.7)
Applicants:
UNIVERSITE LIBRE DE BRUXELLES [BE/BE]; Avenue F.D. Roosevelt 50 CP161 B-1050 Bruxelles, BE
Inventors:
RASPE, Eric; BE
ROGER, Pierre; BE
COULONVAL, Katia; BE
PATERNOT, Sabine; BE
BISTEAU, Xavier; BE
Agent:
VANHALST, Koen Victor Rachel; De Clercq & Partners E. Gevaertdreef 10a B-9830 Sint-Martens-Latem, BE
Priority Data:
PCT/EP2012/06734305.09.2012EP
Title (EN) IMMOBILISED CYCLIN-DEPENDENT KINASE 4 FUSION PROTEINS AND USES THEREOF
(FR) PROTÉINES DE FUSION IMMOBILISÉES DE TYPE KINASE 4 CYCLINE-DÉPENDANTE ET LEURS UTILISATIONS
Abstract:
(EN) The present invention concerns an in vitro assay for determining the activation status of endogenous CDK4 in eukaryotic cells, said assay comprising the steps of: providing eukaryotic cells maintained in a quiescent state, said eukaryotic cells comprising a cyclin D/CDK4 fusion protein, wherein the CDK4 part of the fusion protein is present in a hypophosphorylated form; inducing proliferation of said eukaryotic cells; isolating the cyclin D/CDK4 fusion protein from said eukaryotic cells; and measuring the activation status of said isolated cyclin D/CDK4 fusion protein, thereby determining the activation stats of endogenous CDK4 in said eukaryotic cells.
(FR) Cette invention concerne un dosage in vitro permettant de déterminer l'état d'activation des CDK4 endogènes dans des cellules eucaryotes, ledit dosage comprenant les étapes suivantes : utilisation de cellules eucaryotes maintenues à l'état quiescent, lesdites cellules eucaryotes comprenant une protéine de fusion cycline D/CDK4, la partie CDK4 de la protéine de fusion étant présente sous une forme hypophosphorylée; induction de la prolifération desdites cellules eucaryotes; isolation de la protéine de fusion cycline D/CDK4 desdites cellules eucaryotes; et mesure de l'état d'activation de ladite protéine de fusion cycline D/CDK4 isolée, pour déterminer ainsi l'état d'activation des CDK4 endogènes dans lesdites cellules eucaryotes.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)