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1. (WO2014028295) USE OF A MAIZE UNTRANSLATED REGION FOR TRANSGENE EXPRESSION IN PLANTS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2014/028295 International Application No.: PCT/US2013/054102
Publication Date: 20.02.2014 International Filing Date: 08.08.2013
IPC:
C12N 15/82 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
Applicants:
DOW AGROSCIENCES LLC [US/US]; 9330 Zionsville Road Indianapolis, IN 46268, US
KUMAR, Sandeep [IN/US]; US
GUPTA, Manju [US/US]; US
ALABED, Diaa [US/US]; US
Inventors:
KUMAR, Sandeep; US
GUPTA, Manju; US
ALABED, Diaa; US
Agent:
LEE, Yung-Hui; Dow AgroSciences LLC 9330 Zionsville Rd Indianapolis, IN 46268, US
Priority Data:
61/684,25517.08.2012US
Title (EN) USE OF A MAIZE UNTRANSLATED REGION FOR TRANSGENE EXPRESSION IN PLANTS
(FR) UTILISATION D'UNE RÉGION NON-TRADUITE DU MAÏS POUR L'EXPRESSION D'UN TRANSGÈNE DANS DES PLANTES
Abstract:
(EN) Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3' untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.
(FR) L'invention concerne des procédés, des vecteurs et des constructions de gène pour améliorer l'expression d'une séquence d'acide nucléique recombinante dans des plantes transgéniques et des tissus végétaux transgéniques. Selon la présente invention, des séquences d'acide nucléique sont obtenues et/ou issues à partir des régions 3' non-traduites de gènes codant pour des protéines ubiquitines et modifiées pour flanquer des parties respectives d'une région codante choisie d'un vecteur. La construction de vecteur peut être introduite dans des plantes et/ou tissus végétaux par l'intermédiaire de procédures classiques ou de ciblage de gènes, conduisant à l'expression accrue de la région codante choisie. Dans certains modes de réalisation, la région codante choisie est un gène chimérique ou un fragment de gène exprimant une ou plusieurs protéines connues comme attribuant un taux d'activité insecticide à une plante transgénique et/ou un tissu végétal transgénique.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
CA2884256AR092152CN104662156EP2885412US20150203856IL237206
JP2015524672KR1020150041788RU2015109129AU2013302947IN512/DELNP/2015BR112015002918