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1. WO2014014347 - SINGLE MOLECULE PROTEIN SEQUENCING

Publication Number WO/2014/014347
Publication Date 23.01.2014
International Application No. PCT/NL2013/050537
International Filing Date 15.07.2013
Chapter 2 Demand Filed 10.02.2014
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/487 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
G01N 33/58 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
CPC
C12Q 1/37
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
37involving peptidase or proteinase
G01N 2333/952
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
90Enzymes; Proenzymes
914Hydrolases (3)
948acting on peptide bonds (3.4)
95Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
952derived from bacteria
G01N 2458/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2458Labels used in chemical analysis of biological material
G01N 33/48721
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
487of liquid biological material
48707by electrical means
48721Investigating individual macromolecules, e.g. by translocation through nanopores
G01N 33/582
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
582with fluorescent label
G01N 33/6803
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
Applicants
  • TECHNISCHE UNIVERSITEIT DELFT [NL]/[NL]
Inventors
  • JOO, Chirlmin
  • DEKKER, Cornelis
  • VAN GINKEL, Hendrika Geertruida Theodora Maria
  • MEYER, Anne Sara
Agents
  • ELLENS, Andries
Priority Data
200919116.07.2012NL
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SINGLE MOLECULE PROTEIN SEQUENCING
(FR) SÉQUENÇAGE D'UNE PROTÉINE À MOLÉCULE UNIQUE
Abstract
(EN)
The invention provides a device for determining the type of protein in a liquid, the device comprising (a) an immobilized ATP dependent protease based molecular transporter machine configured to guide a protein that is functionalized with labels through a detection area of a detector, (b) said detector, configured to detect a signal as function of the labels of the labelled amino acids, (c) a processor unit, configured to identify from the detector signal a sequence of amino acids of the functionalized protein, wherein the processor unit is further configured to compare the identified sequence of amino acids with the occurrence of such sequence in a database of proteins and to identify the type of protein.
(FR)
L'invention concerne un dispositif pour la détermination du type de protéine dans un liquide, le dispositif comprenant (a) une machine de transporteur moléculaire sur la base d'une protéase dépendante de l'ATP immobilisée, configurée pour guider une protéine qui est fonctionnalisée par des marqueurs par l'intermédiaire d'une région de détection d'un détecteur, (b) ledit détecteur, configuré pour détecter un signal en tant que fonction des marqueurs des acides aminés marqués, (c) une unité de traitement, configurée pour identifier à partir de signal du détecteur une séquence d'acides aminés de la protéine fonctionnalisée, l'unité de traitement étant en outre configurée pour comparer la séquence identifiée d'acides aminés avec l'occurrence d'une telle séquence dans une base de données de protéines et pour identifier le type de protéine.
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