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1. WO2014011723 - GENERATION OF NATIVE RECOMBINANT SECRETED HUMAN ENDOPLASMIC RETICULUM CHAPERONES BY USING THEIR NATIVE SIGNAL SEQUENCES IN YEAST EXPRESSION SYSTEMS

Publication Number WO/2014/011723
Publication Date 16.01.2014
International Application No. PCT/US2013/049843
International Filing Date 10.07.2013
IPC
C12P 21/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
CPC
C07K 14/47
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
C07K 14/4702
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
4701not used
4702Regulators; Modulating activity
C07K 14/4728
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
4701not used
4728Calcium binding proteins, e.g. calmodulin
C12N 15/81
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
C12N 9/90
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
90Isomerases (5.)
C12P 21/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
Applicants
  • UAB BALTYMAS [LT]/[LT] (AllExceptUS)
  • ČIPLYS, Evaldas [LT]/[LT] (US)
  • SLIBINSKAS, Rimantas [LT]/[LT] (US)
  • SASNAUSKAS, Kęstutis [LT]/[LT] (US)
  • MICHALAK, Marek [CA]/[CA] (US)
  • GOLD, Leslie, Ina [US]/[US] (US)
Inventors
  • ČIPLYS, Evaldas
  • SLIBINSKAS, Rimantas
  • SASNAUSKAS, Kęstutis
  • MICHALAK, Marek
  • GOLD, Leslie, Ina
Agents
  • LYMAN, Beverly, A.
Priority Data
61/670,76812.07.2012US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) GENERATION OF NATIVE RECOMBINANT SECRETED HUMAN ENDOPLASMIC RETICULUM CHAPERONES BY USING THEIR NATIVE SIGNAL SEQUENCES IN YEAST EXPRESSION SYSTEMS
(FR) GÉNÉRATION DE PROTÉINES CHAPERONNES DU RÉTICULUM ENDOPLASMIQUE HUMAINES SÉCRÉTÉES RECOMBINANTES ENDOGÈNES À L'AIDE DE LEURS SÉQUENCES SIGNAL ENDOGÈNES DANS DES SYSTÈMES D'EXPRESSION DE LEVURE
Abstract
(EN)
A method using yeast as a host for production of human ER chaperone proteins, using endogenous signal peptides of intracellular human proteins that are recognized and correctly processed in the yeast cells to subsequently lead to the secretion of the human proteins. The resultant proteins possessed native amino acid sequence and were biologically active. Moreover, secretion allowed simple one-step purification of native recombinant human proteins with high yields.
(FR)
L'invention concerne un procédé utilisant la levure en tant qu'hôte pour la production de protéines chaperonnes d'ER humaines, à l'aide de peptides signal endogènes de protéines humaines intracellulaires qui sont reconnues et transformées correctement dans les cellules de levure pour conduire ultérieurement à la sécrétion des protéines humaines. Les protéines résultantes possédaient une séquence d'acides aminés endogène et étaient biologiquement actives. De plus, la sécrétion permettait une purification d'un seul pas de protéines humaines recombinantes endogènes présentant de hauts rendements.
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