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1. WO2013188582 - KINETIC EXCLUSION AMPLIFICATION OF NUCLEIC ACID LIBRARIES

Publication Number WO/2013/188582
Publication Date 19.12.2013
International Application No. PCT/US2013/045491
International Filing Date 12.06.2013
IPC
C12Q 1/68 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 27/447 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
B01J 19/0046
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
19Chemical, physical or physico-chemical processes in general; Their relevant apparatus
0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
B01J 2219/00585
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00585Parallel processes
B01J 2219/00587
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00585Parallel processes
00587High throughput processes
B01J 2219/00608
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00605the compounds being directly bound or immobilised to solid supports
00608DNA chips
B01J 2219/00621
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00605the compounds being directly bound or immobilised to solid supports
00614Delimitation of the attachment areas
00621by physical means, e.g. trenches, raised areas
B01J 2219/00637
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00605the compounds being directly bound or immobilised to solid supports
00632Introduction of reactive groups to the surface
00637by coating it with another layer
Applicants
  • ILLUMINA, INC. [US]/[US]
Inventors
  • SHEN, Min-Jui Richard
  • BOUTELL, Jonathan Mark
  • STEPHENS, Kathryn M.
  • RONAGHI, Mostafa
  • GUNDERSON, Kevin L.
  • VENKATESAN, Bala Murali
  • BOWEN, M. Shane
  • VIJAYAN, Kandaswamy
Agents
  • MURPHY, John T.
Priority Data
13/783,04301.03.2013US
61/660,48715.06.2012US
61/715,47818.10.2012US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) KINETIC EXCLUSION AMPLIFICATION OF NUCLEIC ACID LIBRARIES
(FR) AMPLIFICATION PAR EXCLUSION CINÉTIQUE DE BANQUES D'ACIDES NUCLÉIQUES
Abstract
(EN) A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
(FR) L'invention concerne un procédé qui comprend (a) l'utilisation d'un réactif d'amplification comprenant un réseau de sites et d'une solution ayant différents acides nucléiques cibles ; (b) la réaction du réactif d'amplification afin de produire des sites d'amplification, ayant chacun une population clonale d'amplicons à partir d'un acide nucléique cible provenant de la solution. La réaction peut comprendre le transport simultané des acides nucléiques vers les sites à une vitesse moyenne de transport, et l'amplification des acides nucléiques qui sont transportés vers les sites à une vitesse moyenne d'amplification, la vitesse d'amplification moyenne dépassant la vitesse moyenne de transport. La réaction peut comprendre la production d'un premier amplicon à partir d'un acide nucléique qui est transporté vers chacun des sites, et la production d'amplicons ultérieurs à partir de l'acide nucléique ou à partir du premier amplicon, la vitesse moyenne à laquelle les amplicons ultérieurs sont générés dépassant la vitesse moyenne à laquelle le premier amplicon est généré.
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