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1. (WO2013160563) METHOD FOR COMPETITIVE ALLELE-SPECIFIC CDNA SYNTHESIS AND DIFFERENTIAL AMPLIFICATION OF THE CDNA PRODUCTS

Pub. No.:    WO/2013/160563    International Application No.:    PCT/FI2013/050470
Publication Date: Fri Nov 01 00:59:59 CET 2013 International Filing Date: Fri Apr 26 01:59:59 CEST 2013
IPC: C12Q 1/68
C12N 15/11
Applicants: HO, Tho Huu
Inventors: HO, Tho Huu
Title: METHOD FOR COMPETITIVE ALLELE-SPECIFIC CDNA SYNTHESIS AND DIFFERENTIAL AMPLIFICATION OF THE CDNA PRODUCTS
Abstract:
The present invention provides a method for the detection of the presence of RNA variants comprising the step of performing a competitive c DNA synthesis comprising a first primer specific to a first RNA variant, a second primer specific to a second RNA variant, an RNA-dependent DNA polymerase, and RNA from said sample as a template, wherein said first primer and said second primer comprise an allele-specific nucleotide portion, a target-specific sequence and tag units with a common sequence and/or a discriminating sequence so that the sequence of said tag units is not complementary to said first or second RNA variant, wherein each of the c DNA products obtained from said competitive c DNA synthesis consists of the sequence of only one primer extended by the sequence complementary to one of the target RNA variants.