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1. (WO2013158092) METHODS AND COMPOSITIONS FOR DUAL EXTRACTION OF PROTEIN AND NUCLEIC ACID

Pub. No.:    WO/2013/158092    International Application No.:    PCT/US2012/034086
Publication Date: Fri Oct 25 01:59:59 CEST 2013 International Filing Date: Thu Apr 19 01:59:59 CEST 2012
IPC: C07K 1/14
C07H 1/06
Applicants: SYNGENTA PARTICIPATIONS AG
YARNALL, Michele Susan
WHINNA, Rachel
WANG, Wenling
HUANG, Jamie
Inventors: YARNALL, Michele Susan
WHINNA, Rachel
WANG, Wenling
HUANG, Jamie
Title: METHODS AND COMPOSITIONS FOR DUAL EXTRACTION OF PROTEIN AND NUCLEIC ACID
Abstract:
The present invention provides a method of isolating nucleic acid and protein from the same biological sample, comprising, in the following order: a) disrupting the biological sample; b) contacting the disrupted biological sample of (a) with a protein lysis buffer that lacks any component that denatures or reduces protein to produce a first lysate; c) centrifuging the first lysate of (b) to produce a first supernatant containing protein and a pellet containing nucleic acid; d) removing the first supernatant of (c), thereby isolating protein from the biological sample; e) contacting the pellet of (d) with nucleic acid lysis buffer to produce a second lysate; f) centrifuging the second lysate of (e) to produce a second supernatant containing nucleic acid; and g) removing the second supernatant of (f), thereby isolating nucleic acid from the same biological sample.