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1. WO2013156720 - METHOD FOR REFINING SQUALENE PRODUCED BY MICROALGAE

Publication Number WO/2013/156720
Publication Date 24.10.2013
International Application No. PCT/FR2013/050812
International Filing Date 15.04.2013
IPC
B01D 11/04 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
11Solvent extraction
04of solutions which are liquid
A23L 1/30 2006.01
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D91; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
1Foods or foodstuffs; Their preparation or treatment
29Modifying nutritive qualities of foods; Dietetic products
30containing additives
A61K 8/97 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
8Cosmetics or similar toilet preparations
18characterised by the composition
96containing materials, or derivatives thereof, of undetermined constitution
97from algae, fungi, lichens or plants; from derivatives thereof
A61Q 19/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
QSPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
19Preparations for care of the skin
C12N 1/12 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
12Unicellular algae; Culture media therefor
C12P 5/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
5Preparation of hydrocarbons
02acyclic
CPC
A23L 17/60
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT
17Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
60Edible seaweed
A23L 33/105
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT
33Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
10using additives
105Plant extracts, their artificial duplicates or their derivatives
A61K 2236/19
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
2236Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
10Preparation or pretreatment of starting material
19involving fermentation using yeast, bacteria or both; enzymatic treatment
A61K 2236/37
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
2236Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
30Extraction of the material
37Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
A61K 2800/10
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
2800Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
10General cosmetic use
A61K 2800/85
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
2800Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Applicants
  • ROQUETTE FRERES [FR]/[FR]
Inventors
  • LOOTEN, Philippe
  • PATINIER, Samuel
  • PERRUT, Michel
  • PERRUT, Vincent
Agents
  • GALLOIS, Valérie
Priority Data
125349616.04.2012FR
125361419.04.2012FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) METHOD FOR REFINING SQUALENE PRODUCED BY MICROALGAE
(FR) PROCÉDÉ DE RAFFINAGE DU SQUALÈNE PRODUIT PAR MICROALGUES
Abstract
(EN)
The invention relates to a method for preparing a composition that is very rich in squalene produced by fermentation of micro-organisms. The method is characterised in that it comprises a purification step selected from the group including: supercritical CO2 extraction in a multi-stage counter-current fractionation column with extract reflux, and short-path molecular distillation.
(FR)
L'invention se rapporte à un procédé de préparation d'une composition à haute richesse en squalène produit par fermentation de microorganismes, caractérisé en ce qu'il comprend une étape de purification choisie dans le groupe constitué de l'extraction par CO2 supercritique sur colonne de fractionnement multiétagée fonctionnant à contre-courant avec reflux d'extrait,et de la distillation moléculaire dite de «court trajet».
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