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1. (WO2013155864) METHOD FOR PRODUCING CORDYCEPIC ACID BY MEANS OF LIQUID FERMENTATION OF CORDYCEPS MILITARIS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2013/155864 International Application No.: PCT/CN2013/000371
Publication Date: 24.10.2013 International Filing Date: 01.04.2013
IPC:
C12P 7/18 (2006.01) ,C12R 1/645 (2006.01) ,C12N 1/14 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
02
containing a hydroxy group
04
acyclic
18
polyhydric
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
645
Fungi
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
Applicants: JINAN YIAN BIOLOGY INSTITUTE[CN/CN]; PAN, Deng NO.108 Huanshanlu,Lixia District Jinan, Shandong 250014, CN
INSTITUTE OF AGRICULTURAL RESOURCES AND ENVIRONMENT, SHANDONG ACADEMY OF AGRICULTURAL SCIENCES[CN/CN]; YAO, Qiang NO.202 Gongyebeilu, Licheng District Jinan, Shandong 250100, CN
Inventors: YAO, Qiang; CN
GONG, Zhiyuan; CN
LIU, Zhaohui; CN
LIU, Chunhua; CN
PAN, Deng; CN
XIAO, Li; CN
ZHAO, Junsheng; CN
LIU, Xiao; CN
HAN, Jiandong; CN
WAN, Luchang; CN
REN, Pengfei; CN
SUN, Tao; CN
YANG, Peng; CN
Agent: JINAN JINDI INTELLECTUAL PROPERTY AGENCY CO., LTD.; NO.27 Shandanan Rd., Licheng District, Jinan Shandong 250100, CN
Priority Data:
201210111512.616.04.2012CN
Title (EN) METHOD FOR PRODUCING CORDYCEPIC ACID BY MEANS OF LIQUID FERMENTATION OF CORDYCEPS MILITARIS
(FR) PROCÉDÉ DE PRODUCTION D'ACIDE CORDYCÉPIQUE AU MOYEN DE LA FERMENTATION LIQUIDE DE CORDYCEPS MILITARIS
(ZH) 一种利用蛹虫草液体发酵生产虫草酸的方法
Abstract:
(EN) Provided is a method for producing cordycepic acid by means of the liquid fermentation of Cordyceps militaris, comprising the steps of: (1) inoculating strains of Cordyceps militaris into the PD liquid fermentation medium, activating the culturing of the strains, then transferring them to a seed fermentation medium and obtaining a seed solution from the seed culture; (2) inoculating the seed solution into a liquid fermentation medium and culturing for 20 - 30 h, then adding an expansin solution and further culturing for 96 - 144 h, isolating and obtaining Cordyceps militaris mycelium; (3) extracting intracellular cordycepic acid of Cordyceps militaris from the Cordyceps militaris mycelium. An expansin is used in the liquid fermentation production of cordycepic acid of Cordyceps militaris, which increases the fermentation yield of cordycepic acid.
(FR) L'invention concerne un procédé de production d'acide cordycépique au moyen de la fermentation liquide de Cordyceps militaris, comprenant les étapes de : (1) inoculation de souches de Cordyceps militaris dans le milieu de fermentation liquide PD, activation de la culture des souches puis transfert de celles-ci dans un milieu de fermentation d'ensemencement et obtention d'une solution d'ensemencement à partir de la culture d'ensemencement; (2) inoculation de la solution d'ensemencement dans un milieu de fermentation liquide et culture pendant 20-30 h, puis addition d'une solution d'expansine et culture supplémentaire pendant 96-144 h, isolement et obtention d'un mycélium de Cordyceps militaris ; (3) extraction d'acide cordycépique intracellulaire de Cordyceps militaris à partir du mycélium de Cordyceps militaris. Une expansine est utilisée dans la production de fermentation liquide de l'acide cordycépique de Cordyceps militaris, ce qui augmente le rendement de fermentation de l'acide cordycépique.
(ZH) 提供一种利用蛹虫草液体发酵生产虫草酸的方法,(1)将蛹虫草菌种接种到PD液体发酵培养基中,进行活化培养,然后转接到种子发酵培养基中,进行种子培养,得种子液;(2)将种子液接种于液体发酵培养基中,液体发酵培养20〜30h,然后加入扩张蛋白溶液,继续培养96〜144h,分离,得到蛹虫草菌丝体;(3)从蛹虫草菌丝体中提取蛹虫草胞内虫草酸。将扩张蛋白应用于蛹虫草的虫草酸液体发酵生产,提高了虫草酸发酵产量。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)