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1. (WO2013155471) METHOD AND APPARATUS FOR QUANTIFYING OXYGEN CONSUMPTION OF MITOCHONDRIA

Pub. No.:    WO/2013/155471    International Application No.:    PCT/US2013/036462
Publication Date: Fri Oct 18 01:59:59 CEST 2013 International Filing Date: Sat Apr 13 01:59:59 CEST 2013
IPC: G01N 33/497
C12Q 1/02
Applicants: THE TRUSTEES OF DARTMOUTH COLLEGE
Inventors: SPRINGETT, Roger
Title: METHOD AND APPARATUS FOR QUANTIFYING OXYGEN CONSUMPTION OF MITOCHONDRIA
Abstract:
A system for measuring oxygen consumption uses a chamber holding culture medium with a biological material. A resistively gas-permeable membrane has a first side contacting culture medium in the chamber and a first sensor for measuring oxygen in the culture medium. A second sensor measures oxygen in gas adjacent a second side of the membrane. The sensors provide data to a processor having a memory with machine readable instructions for determining oxygen consumption from the data. Alternatively, a method of measuring oxygen flux begins with placing biological material, with fluid, in the chamber; exposing the fluid to a resistively-permeable membrane with oxygen-containing gas on a second side of the membrane; measuring oxygen in the fluid; measuring oxygen in the gas, and calculating the oxygen flux of the biological material from an oxygen permeability constant of the membrane and a difference between the oxygen concentrations of the fluid and gas.