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1. WO2013153361 - GENE EXPRESSSION FROM MITOTICALLY STABLE EPISOMES

Publication Number WO/2013/153361
Publication Date 17.10.2013
International Application No. PCT/GB2013/050795
International Filing Date 27.03.2013
IPC
C12N 15/67 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12N 15/867 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
867Retroviral vectors
CPC
C12N 15/67
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12N 15/79
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
C12N 15/86
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2710/22022
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2710dsDNA Viruses
00011dsDNA Viruses
22011Polyomaviridae, e.g. polyoma, SV40, JC
22022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
C12N 2740/16043
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2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
16011Human Immunodeficiency Virus, HIV
16041Use of virus, viral particle or viral elements as a vector
16043viral genome or elements thereof as genetic vector
C12N 2800/108
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2800Nucleic acids vectors
10Plasmid DNA
108episomal vectors
Applicants
  • ROYAL HOLLOWAY AND BEDFORD NEW COLLEGE [GB]/[GB]
Inventors
  • DICKSON, George
  • YANEZ-MUNOS, Rafael
  • KYMALAINEN, Hanna
Agents
  • HUTTER, Anton
Priority Data
1206455.612.04.2012GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) GENE EXPRESSSION FROM MITOTICALLY STABLE EPISOMES
(FR) EXPRESSION GÉNIQUE À PARTIR D'ÉPISOMES MITOTIQUEMENT STABLES
Abstract
(EN)
The invention provides a method of expressing a transgene in a host cell. The method comprises exposing a host cell, which comprises a genetic construct comprising a transgene, to conditions which substantially block mitosis in the host cell, such that the construct forms a mitotically stable episome resulting in expression of the transgene. The invention extends to the use of such constructs for expressing transgenes in a range of different medical and heterologous protein production applications.
(FR)
Cette invention concerne un procédé d'expression d'un transgène dans une cellule hôte, le procédé comprenant l'exposition de la cellule hôte, qui contient un produit de recombinaison génétique comprenant un transgène, à des conditions qui bloquent sensiblement la mitose dans la cellule hôte, de façon que le produit de recombinaison forme un épisome mitotiquement stable permettant l'expression du transgène. Cette invention s'étend à l'utilisation de ces produits de recombinaison pour exprimer des transgènes dans tout un éventail d'applications de production de protéines à visée médicale et hétérologues différentes.
Also published as
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