Processing

Please wait...

Settings

Settings

Goto Application

1. WO2013150142 - METHOD FOR THE PREPARATION OF A STRAIN- ADAPTED VACCINE

Publication Number WO/2013/150142
Publication Date 10.10.2013
International Application No. PCT/EP2013/057216
International Filing Date 05.04.2013
IPC
A61K 35/74 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
66Microorganisms or materials therefrom
74Bacteria
A61K 39/02 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
02Bacterial antigens
CPC
A61K 2039/543
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
54characterised by the route of administration
541Mucosal route
543intranasal
A61K 2039/552
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
55characterised by the host/recipient, e.g. newborn with maternal antibodies
552Veterinary vaccine
A61K 2039/55555
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
555characterised by a specific combination antigen/adjuvant
55511Organic adjuvants
55555Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
A61K 2039/6068
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
60characteristics by the carrier linked to the antigen
6031Proteins
6068Other bacterial proteins, e.g. OMP
A61K 2039/625
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
62characterised by the link between antigen and carrier
625binding through the biotin-streptavidin system or similar
A61K 35/74
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
66Microorganisms or materials therefrom
74Bacteria
Applicants
  • LUDWIG-MAXIMILIANS-UNIVERSITÄT MÜNCHEN [DE]/[DE]
Inventors
  • SCHUBERT, Sören
  • WIESER, Andreas
Agents
  • VOSSIUS & PARTNER
Priority Data
12163468.705.04.2012EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR THE PREPARATION OF A STRAIN- ADAPTED VACCINE
(FR) PROCÉDÉ POUR LA PRÉPARATION D'UN VACCIN ADAPTÉ À UNE SOUCHE
Abstract
(EN)
The present invention relates to a method for the preparation of a strain-adapted vaccine specific for a bacterial strain, comprising the steps of: (a) genetically engineering a bacterial strain obtained from a subject, wherein said genetic engineering comprises introducing a nucleic acid molecule encoding a fusion protein, wherein the fusion protein comprises a bacterial membrane protein fused to at least one affinity tag, (b) growing the genetically engineered bacterial strain obtained in step (a) in solution, (c) isolating membrane vesicles from the growth culture of step (b) by affinity purification using the affinity tag, and (d) formulating the membrane vesicles isolated in step (c) into a strain-adapted vaccine. The present invention further relates to a nucleic acid molecule encoding a fusion protein comprising a bacterial membrane protein fused to at least one affinity tag and a kit comprising said fusion protein.
(FR)
La présente invention concerne un procédé pour la préparation d'un vaccin spécifiquement adapté à une souche bactérienne, comprenant les étapes suivantes : (a) modification génétique d'une souche bactérienne obtenue à partir d'un sujet, ladite modification génétique comprenant l'introduction d'une molécule d'acide nucléique codant pour une protéine de fusion, la protéine de fusion comprenant une protéine de membrane bactérienne fusionnée à au moins un marqueur d'affinité, (b) la culture de la souche bactérienne génétiquement modifiée obtenue à l'étape (a) en solution, (c) isolation de vésicules membranaires à partir de la culture de croissance de l'étape (b) par purification par affinité à l'aide du marqueur d'affinité, et (d) formulation des vésicules membranaires isolées à l'étape (c) en un vaccin adapté à une souche. La présente invention concerne en outre une molécule d'acide nucléique codant pour une protéine de fusion comprenant une protéine de membrane bactérienne fusionnée à au moins un marqueur d'affinité et un kit comprenant ladite protéine de fusion.
Also published as
Latest bibliographic data on file with the International Bureau