WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Options
Query Language
Stem
Sort by:
List Length
Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2013146940) MODIFIED LEUCINE DEHYDROGENASE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2013/146940 International Application No.: PCT/JP2013/059124
Publication Date: 03.10.2013 International Filing Date: 27.03.2013
IPC:
C12N 15/09 (2006.01) ,C12N 9/06 (2006.01) ,C12Q 1/32 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
06
acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
26
involving oxidoreductase
32
involving dehydrogenase
Applicants: AJINOMOTO CO., INC.[JP/JP]; 15-1, Kyobashi 1-chome, Chuo-ku, Tokyo 1048315, JP
Inventors: HOSHINO, Wataru; JP
KODAMA, Yuya; JP
MIZUKOSHI, Toshimi; JP
TAGAMI, Uno; JP
Agent: SAKAI, Hiroaki; Sakai International Patent Office, Kasumigaseki Building, 2-5, Kasumigaseki 3-chome, Chiyoda-ku, Tokyo 1006020, JP
Priority Data:
2012-08277730.03.2012JP
Title (EN) MODIFIED LEUCINE DEHYDROGENASE
(FR) LEUCINE DÉSHYDROGÉNASE MODIFIÉE
(JA) 改変ロイシン脱水素酵素
Abstract:
(EN) The present invention provides a means and method useful in the measurement of the concentration of all branched-chain amino acids. Specifically, the invention provides a modified enzyme in which at least one amino acid residue has been varied so as to improve the properties of leucine dehydrogenase as relates to the measurement of all branched-chain amino acids (e.g., substrate specificity of leucine dehydrogenase relative to all branched-chain amino acids, activity of leucine dehydrogenase relative to a given branched-chain amino acid, and heat stability of leucine dehydrogenase); a method for analyzing all branched-chain amino acids including measuring all branched-chain amino acids contained in a test sample using this modified enzyme, and the like.
(FR) Cette invention concerne un moyen et un procédé utiles pour mesurer la concentration de tous les acides aminés à chaîne ramifiée. Plus spécifiquement, cette invention concerne une enzyme modifiée contenant au moins un résidu acide aminé qui a été modifié de façon à améliorer les propriétés de la leucine déshydrogénase qui se rapportent à la mesure de tous les acides aminés à chaîne ramifiée (par ex., spécificité de substrat de la leucine déshydrogénase par rapport à tous les acides aminés à chaîne ramifiée, activité de la leucine déshydrogénase par rapport à un acide aminé à chaîne ramifiée donné, et thermostabilité de la leucine déshydrogénase) ; un procédé pour analyser tous les acides aminés à chaîne ramifiée comprenant la mesure de tous les acides aminés à chaîne ramifiée contenus dans un échantillon d'essai à l'aide de cette enzyme modifiée, et autres.
(JA)  本発明は、総分岐鎖アミノ酸濃度の測定に有用な手段および方法を提供する。具体的には、本発明は、総分岐鎖アミノ酸の測定に関連するロイシン脱水素酵素の特性(例、総分岐鎖アミノ酸に対するロイシン脱水素酵素の基質特異性、任意の分岐鎖アミノ酸に対するロイシン脱水素酵素の活性、およびロイシン脱水素酵素の熱安定性)を改善するようにその少なくとも1つのアミノ酸残基を変異させた、改変酵素;および当該改変酵素を用いて被検試料中に含まれる総分岐鎖アミノ酸を測定することを含む、総分岐鎖アミノ酸の分析方法などを提供する。
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)