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1. (WO2013144722) METHODS FOR DETECTION OF ANTI-CYTOMEGALOVIRUS NEUTRALIZING ANTIBODIES

Pub. No.:    WO/2013/144722    International Application No.:    PCT/IB2013/001021
Publication Date: Fri Oct 04 01:59:59 CEST 2013 International Filing Date: Thu Mar 28 00:59:59 CET 2013
IPC: C12Q 1/70
G01N 33/52
G01N 33/564
Applicants: VARIATION BIOTECHNOLOGIES, INC.
Inventors: ANDERSON, David, E.
BOZIC, Jasminka
ONTSOUKA, Barthelemy
Title: METHODS FOR DETECTION OF ANTI-CYTOMEGALOVIRUS NEUTRALIZING ANTIBODIES
Abstract:
The present disclosure provides methods useful for determining levels of HCMV infection in host cells and, by extension, determining levels of neutralizing antibodies present in a sample. The present disclosure encompasses the recognition that HCMV viruses that have a fluorescent moiety permit detection of viral infection (e.g., by assessing fluorescence in cells after contacting the host cell with the virus). In some embodiments, levels of HCMV infection are determined by fluorescence detection where the virus has been preincubated with a test sample (e.g., a serum sample) from a subject. In some embodiments, the subject has been administered a candidate HCMV vaccine.