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1. (WO2013124309) DIRECT REPROGRAMMING OF SOMATIC CELLS INTO NEURAL STEM CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2013/124309 International Application No.: PCT/EP2013/053366
Publication Date: 29.08.2013 International Filing Date: 20.02.2013
IPC:
C12N 5/079 (2010.01) ,C12N 5/0797 (2010.01) ,C12N 5/071 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
079
Neural cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
079
Neural cells
0797
Stem cells; Progenitor cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
Applicants:
MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V. [DE/DE]; Hofgartenstr. 8 80539 München, DE
Inventors:
HAN, Dong Wook; KR
SCHÖLER, Hans R.; DE
TAPIA, Natalia; DE
Agent:
VOSSIUS & PARTNER; Siebertstraße 4 81675 München, DE
Priority Data:
12156272.220.02.2012EP
Title (EN) DIRECT REPROGRAMMING OF SOMATIC CELLS INTO NEURAL STEM CELLS
(FR) REPROGRAMMATION DIRECTE DE CELLULES SOMATIQUES EN CELLULES SOUCHES NEURALES
Abstract:
(EN) The present invention relates to a method for producing induced neural stem cells (iNSC), comprising (a) introducing into somatic cells (i) a Sox family member, (ii) a Klf family member; (iii) a Myc family member; and (iv) a POL) family member, wherein the POU family member is not Oct4; and (b) culturing the cells for at least about 2 days. The present invention further relates to induced neural stem cells obtainable by the method of the invention, as well as their use in producing induced pluripotent stem cells. Furthermore, the present invention relates to the induced neural stem cells for use in medicine or medical/pharmaceutical research, in particular for use in the treatment of a disease or disorder associated with a reduced number of neurons as compared to healthy subjects.
(FR) La présente invention concerne un procédé pour la production de cellules souches neurales induites (iNSC), comprenant (a) l'introduction dans des cellules somatiques (i) d'un membre de la famille Sox, (ii) d'un membre de la famille Klf, (iii) d'un membre de la famille Myc, et (iv) d'un membre de la famille POU, le membre de la famille POU n'étant pas Oct4; et (b) la mise en culture des cellules pendant au moins environ 2 jours. La présente invention concerne en plus les cellules souches neurales induites qu'on peut obtenir par le procédé de l'invention, ainsi que leur utilisation dans la production de cellules souches pluripotentes induites. De plus, la présente invention concerne les cellules souches neurales induites pour l'utilisation en médecine ou dans la recherche médicale/pharmaceutique, en particulier pour l'utilisation dans le traitement d'une maladie ou d'un trouble associé à un nombre réduit de neurones par comparaison aux sujets sains.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)