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1. (WO2013123379) NANOPORE SENSOR FOR ENZYME-MEDIATED PROTEIN TRANSLOCATION

Pub. No.:    WO/2013/123379    International Application No.:    PCT/US2013/026414
Publication Date: Fri Aug 23 01:59:59 CEST 2013 International Filing Date: Sat Feb 16 00:59:59 CET 2013
IPC: C12Q 1/00
C12M 1/34
C07H 21/04
Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors: NIVALA, Jeffrey, M.
MARKS, Douglas, B.
AKESON, Mark, A.
Title: NANOPORE SENSOR FOR ENZYME-MEDIATED PROTEIN TRANSLOCATION
Abstract:
Described herein is a device and method for translocating a protein through a nanopore and monitoring electronic changes caused by different amino acids in the protein. The device comprises a nanopore in a membrane, an amplifier for providing a voltage between the cis side and trans side of the membrane, and an NTP driven unfoldase which processed the protein to be translocated. The exemplified unfoldase is the ClpX unfoldase from E. coli.