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1. (WO2013121228) ENDONUCLEASES

Pub. No.:    WO/2013/121228    International Application No.:    PCT/GB2013/050387
Publication Date: Fri Aug 23 01:59:59 CEST 2013 International Filing Date: Tue Feb 19 00:59:59 CET 2013
IPC: C12N 9/22
C12N 9/99
C12Q 1/68
Applicants: BIOTEC PHARMACON ASA
GARDNER, Rebecca
Inventors: LANES, Olav
HAVDALEN, Linda
SOLSTAD, Terese
LORENTZEN, Marit
ALTERMARK, Bjørn
LEIROS, Ingar
HELLAND, Ronny
Title: ENDONUCLEASES
Abstract:
The present invention provides an endonuclease I or enzymatically active fragment thereof wherein said endonuclease I has the sequence of SEQ ID No. 4 or a sequence which is at least 70% identical thereto and wherein the amino acid residue which is immediately N-terminal of the FYCGC pentapeptide motif has been substituted with a residue which is negatively charged as well as nucleic acid molecules encoding these enzymes and methods of removing contaminating polynucleotides from a sample using these enzyme.