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Machine translation
1. (WO2013119784) METHODS OF SEQUENCING NUCLEIC ACIDS USING NANOPORES AND ACTIVE KINETIC PROOFREADING
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2013/119784    International Application No.:    PCT/US2013/025106
Publication Date: 15.08.2013 International Filing Date: 07.02.2013
IPC:
C12Q 1/68 (2006.01), C12N 15/11 (2006.01)
Applicants: BROWN UNIVERSITY [US/US]; Horace Mann Building 47 George Street 3rd Floor Providence, Rhode Island 02912 (US)
Inventors: LING, Xinsheng, Sean; (US)
Agent: GUTERMAN, Sonia K.; Lawson & Weitzen, LLP 88 Black Falcon Avenue Suite 345 Boston, Massachusetts 02210 (US)
Priority Data:
61/596,491 08.02.2012 US
Title (EN) METHODS OF SEQUENCING NUCLEIC ACIDS USING NANOPORES AND ACTIVE KINETIC PROOFREADING
(FR) PROCÉDÉS DE SÉQUENÇAGE D'ACIDES NUCLÉIQUES À L'AIDE DE NANOPORES ET CORRECTION D'ÉPREUVES CINÉTIQUE ACTIVE
Abstract: front page image
(EN)Transient hybridization of di-nucleotides and/or tri-nucleotides to a template single stranded DNA is measured by passage of the complex through a nanopore, such that decreases in ionic current indicate the presence and locations of the bound di-nucleotide or tri-nucleotide to the single stranded DNA. Repeated analyses with all of the members of each di-nucleotides or tri-nucleotides set yield a sequence of the DNA.
(FR)L'invention concerne une hybridation transitoire de di-nucléotides et/ou tri-nucléotides à une matrice d'ADN simple brin qui est mesurée par le passage du complexe à travers un nanopore, de telle sorte que des diminutions d'un courant ionique indiquent la présence et les emplacements du di-nucléotide ou du tri-nucléotide lié à l'ADN simple brin. Des analyses répétées avec tous les membres de chaque ensemble de di-nucléotides ou de tri-nucléotides génèrent une séquence de l'ADN.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)