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1. (WO2013117122) METHOD FOR PURIFYING ATOSIBAN
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2013/117122 International Application No.: PCT/CN2013/070505
Publication Date: 15.08.2013 International Filing Date: 16.01.2013
IPC:
C07K 1/20 (2006.01) ,C07K 7/16 (2006.01) ,C07K 7/06 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
14
Extraction; Separation; Purification
16
by chromatography
20
Partition-, reverse-phase or hydrophobic interaction chromatography
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
7
Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
04
Linear peptides containing only normal peptide links
16
Oxytocins; Vasopressins; Related peptides
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
7
Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
04
Linear peptides containing only normal peptide links
06
having 5 to 11 amino acids
Applicants:
深圳翰宇药业股份有限公司 HYBIO PHARMACEUTICAL CO., LTD. [CN/CN]; 中国广东省深圳市 南山区高新技术工业园中区翰宇生物医药园办公大楼四层 Hybio Medicine Park No.37 KeJic, Str.2nd, Hi-Tech Industrial Park Shenzhen, Guangdong 518057, CN
Inventors:
赵忠卫 ZHAO, Zhongwei; CN
刘建 LIU, Jian; CN
马亚平 MA, Yaping; CN
袁建成 YUAN, Jiancheng; CN
Agent:
深圳市深佳知识产权代理事务所(普通合伙) SHENPAT INTELLECTUAL PROPERTY AGENCY; 中国广东省深圳市 国贸大厦15楼西座1521室 Room 1521 West Block, Guomao Building Shenzhen, Guangdong 518014, CN
Priority Data:
201210028890.809.02.2012CN
Title (EN) METHOD FOR PURIFYING ATOSIBAN
(FR) PROCÉDÉ DE PURIFICATION DE L'ATOSIBAN
(ZH) 一种阿托西班的纯化方法
Abstract:
(EN) The present invention belongs to the technical field of pharmaceutical chemistry.Disclosed is a method for purifying atosiban. The purification method of the present invention comprises: treating the solution of the crude peptide of atosiban with a ferric ion salt as a reducing agent, removing the impurities that are not completely oxidized in the solution, subsequently purifying the solution of the crude peptide of atosiban with high-performance liquid chromatography, and then preparing the purified product of the atosiban by converting same to a salt from the resulting purified atosiban perchlorate using reversed-phase high-performance liquid chromatography. The purification method of the present invention is operationally simple and feasible, and the prepared purified peptide of the atosiban has a high level of purity; meanwhile, the purified peptide of the atosiban produced through the method of the present invention has a high yield, with greater than 1000 g of purified peptide of atosiban being able to be obtained in a batch, which is sufficient to meet industrial requirements.
(FR) La présente invention concerne le domaine technique de la chimie pharmaceutique. L'invention concerne un procédé de purification de l'atosiban. Le procédé de purification selon la présente invention consiste à traiter la solution du peptide d'atosiban brut avec un sel d'ion ferrique comme réducteur, éliminer les impuretés qui ne sont pas totalement oxydées dans la solution, purifier la solution du peptide d'atosiban brut au moyen d'une chromatographie liquide haute performance, puis préparer le produit purifié de l'atosiban par la conversion de celui-ci en sel issu du perchlorate d'atosiban purifié obtenu à l'aide d'une chromatographie liquide haute performance en phase inverse. Le procédé de purification selon la présente invention est simple à mettre en œuvre et faisable, et le peptide purifié préparé de l'atosiban présente un niveau élevé de pureté ; dans le même temps, le peptide purifié de l'atosiban produit par le procédé selon la présente invention présente un haut rendement, plus de 1 000 g de peptide d'atosiban purifié pouvant être obtenus dans un lot, ce qui suffit à satisfaire aux exigences industrielles.
(ZH) 本发明属于药物化学技术领域,公开了一种阿托西班的纯化方法。本发明所述纯化方法以三价铁离子盐作为还原剂对阿托西班粗肽溶液进行处理,除去其中的未氧化完全的杂质,之后利用高效液相色谱法纯化阿托西班粗肽溶液,然后利用反相高效液相色谱将纯化制得的阿托西班高氯酸盐转盐制得阿托西班纯品。本发明所述纯化方法操作简单可行,制得的阿托西班纯品肽纯度高,同时本发明所述方法阿托西班纯品肽收率高,一批次可获得1000g以上的阿托西班纯品肽,足以满足产业化要求。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)