Search International and National Patent Collections

1. (WO2013114859) CELL COLLECTION METHOD

Pub. No.:    WO/2013/114859    International Application No.:    PCT/JP2013/000462
Publication Date: Fri Aug 09 01:59:59 CEST 2013 International Filing Date: Wed Jan 30 00:59:59 CET 2013
IPC: C12N 1/02
C12M 1/00
C12M 3/00
Applicants: TOYO SEIKAN GROUP HOLDINGS, LTD.
東洋製罐グループホールディングス株式会社
Inventors: TANAKA, Satoshi
田中 郷史
SUENAGA, Ryo
末永 亮
KUNINORI, Masahiro
國則 正弘
OTA, Kyohei
太田 恭平
Title: CELL COLLECTION METHOD
Abstract:
[Problem] To enable the improvement in cell collection efficiency without using any novel culture medium for washing purposes or any novel washing solution. [Solution] A method for collecting cells that are cultured using a cell culture kit comprising at least a culture vessel for culturing cells therein, a cell collection vessel for collecting the cells after culturing and a waste solution vessel for collecting the culture medium after culturing wherein the culture vessel, the cell collection vessel and the waste solution vessel are connected to one another through a conduit pipe, said method comprising: a supernatant discharge step of transporting a culture supernatant that has been cultured from the culture vessel into the waste solution vessel; a cell collection step of transporting the concentrated cell suspension from the culture vessel into the cell collection vessel; a supernatant return step of returning a portion of the culture supernatant that has been discharged into the waste solution vessel into the culture vessel to disperse the cells remaining in the culture vessel in the cell suspension; and a recollection step of transferring again the cell suspension in which the remaining cells have been dispersed from the culture vessel into the cell collection vessel.