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|1. (WO2013114859) CELL COLLECTION METHOD|
|Applicants:||TOYO SEIKAN GROUP HOLDINGS, LTD.
|Title:||CELL COLLECTION METHOD|
[Problem] To enable the improvement in cell collection efficiency without using any novel culture medium for washing purposes or any novel washing solution. [Solution] A method for collecting cells that are cultured using a cell culture kit comprising at least a culture vessel for culturing cells therein, a cell collection vessel for collecting the cells after culturing and a waste solution vessel for collecting the culture medium after culturing wherein the culture vessel, the cell collection vessel and the waste solution vessel are connected to one another through a conduit pipe, said method comprising: a supernatant discharge step of transporting a culture supernatant that has been cultured from the culture vessel into the waste solution vessel; a cell collection step of transporting the concentrated cell suspension from the culture vessel into the cell collection vessel; a supernatant return step of returning a portion of the culture supernatant that has been discharged into the waste solution vessel into the culture vessel to disperse the cells remaining in the culture vessel in the cell suspension; and a recollection step of transferring again the cell suspension in which the remaining cells have been dispersed from the culture vessel into the cell collection vessel.