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1. (WO2013113504) METHOD FOR THE GENERATION AND CULTIVATION OF A PLANT CELL PACK
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2013/113504 International Application No.: PCT/EP2013/000296
Publication Date: 08.08.2013 International Filing Date: 31.01.2013
IPC:
C12N 15/82 (2006.01) ,C12N 11/12 (2006.01) ,C12P 21/02 (2006.01) ,A01H 4/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11
Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02
Enzymes or microbial cells being immobilised on or in an organic carrier
10
the carrier being a carbohydrate
12
Cellulose or derivatives thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4
Plant reproduction by tissue culture techniques
Applicants:
FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V. [DE/DE]; Hansastraße 27C 80686 München, DE
Inventors:
RADEMACHER, Thomas; DE
Agent:
STÜRKEN, Joachim; Patentanwaltsgesellschaft mbH Kirchenhölzle 18 79104 Freiburg, DE
Priority Data:
61/592,78031.01.2012US
EP 12 000 618.431.01.2012EP
Title (EN) METHOD FOR THE GENERATION AND CULTIVATION OF A PLANT CELL PACK
(FR) PROCÉDÉ DE GÉNÉRATION ET DE CULTURE D'UN BLOC CELLULAIRE VÉGÉTALE
Abstract:
(EN) The present invention relates to the generation and cultivation of plant cell material in the form of a non-tissue multilayer cell pack and its use for the accumulation or harvesting of a desired product. In particular, the invention provides a method for the generation of plant cell material in the form of a medium- deprived, porous structured and non-tissue multilayer cell pack and for the subsequent maintenance of said cell pack, comprising the steps of (i) providing a cell pack by separating cells from a plant cell suspension culture, wherein the content of the liquid comprised by the cell pack is reduced and adjusted to correspond to a cell pack density between 0.1 and 0.9 g wet cell weight per cm3, thereby establishing the medium-deprived and porous structured nature of said cell pack, and (ii) incubating said medium-deprived and porous structured cell pack in a non-liquid environment under a relative humidity of 50 to 100 %.
(FR) La présente invention concerne la génération et la culture d'une matière cellulaire végétale sous la forme d'un bloc cellulaire multicouche non-tissulaire et son utilisation pour l'accumulation ou la récolte d'un produit souhaité. En particulier, l'invention concerne un procédé de génération de matière cellulaire végétale sous la forme d'un bloc cellulaire multicouche non tissulaire privé de milieu, à structure poreuse et destiné à l'entretien ultérieur dudit bloc cellulaire, comprenant les étapes consistant à (i) fournir un bloc cellulaire en séparant des cellules d'une culture de suspension cellulaire végétale, le contenu du liquide compris par le bloc cellulaire étant réduit et ajusté pour correspondre à une densité bloc cellulaire entre 0,1 et 0,9 g en poids cellulaire humide par cm3, ce qui permet d'établir la nature à structure poreuse, privée de milieu dudit bloc cellulaire, et (ii) incuber ledit bloc cellulaire à structure poreuse et privée de milieu dans un environnement non-liquide sous une humidité relative de 50 à 100 %.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)