Search International and National Patent Collections

1. (WO2013113369) CELL BASED SENSOR

Pub. No.:    WO/2013/113369    International Application No.:    PCT/EP2012/051530
Publication Date: Fri Aug 09 01:59:59 CEST 2013 International Filing Date: Wed Feb 01 00:59:59 CET 2012
IPC: G01N 33/50
Applicants: CANVAX BIOTECH S.L
PAZ ROJAS, Elier
GARCÍA MACEIRA, Fé Isabel
LUNA GUERRERO, Verónica Inmaculada
MONTERO PEÑALVO, María Gracia
GARCÍA MACEIRA, Tania
MORALES MARTÍNEZ, José Andrés
ARAGÓN GÓMEZ, Ana Belén
QUESADA MOLINA, Ana
MÁRQUEZ MORALES, Aurora María
Inventors: PAZ ROJAS, Elier
GARCÍA MACEIRA, Fé Isabel
LUNA GUERRERO, Verónica Inmaculada
MONTERO PEÑALVO, María Gracia
GARCÍA MACEIRA, Tania
MORALES MARTÍNEZ, José Andrés
ARAGÓN GÓMEZ, Ana Belén
QUESADA MOLINA, Ana
MÁRQUEZ MORALES, Aurora María
Title: CELL BASED SENSOR
Abstract:
Cell based sensor. The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a non-protease hydrolase as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis. Said granule stored non protease-hydrolase reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific substrate; absence of toxicity when cell thawing; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection.