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1. (WO2013113196) CULTURE MEDIUM FOR PRIMARY CULTURE OF HIPPOCAMPUS NEURONS OF A NEONATAL RAT AND PREPARATION METHOD AND USE THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2013/113196 International Application No.: PCT/CN2012/075162
Publication Date: 08.08.2013 International Filing Date: 08.05.2012
IPC:
C12N 5/02 (2006.01) ,C12N 5/0797 (2010.01) ,C12N 1/04 (2006.01)
Applicants: THE SECOND AFFILIATED HOSPITAL & YUYING CHILDREN'S HOSPITAL OF WENZHOU MEDICAL UNIVERSITY[CN/CN]; CN
Inventors: SUN, Jing; CN
LIU, Jiaming; CN
Agent: BEIJING HUIZE INTELLECTUAL PROPERTY LAW LLC; ZHANG, Jin A18, Horizon International Tower, No.6, Zhichun Road, Haidian District Beijing 100088, CN
Priority Data:
201210023408.102.02.2012CN
Title (EN) CULTURE MEDIUM FOR PRIMARY CULTURE OF HIPPOCAMPUS NEURONS OF A NEONATAL RAT AND PREPARATION METHOD AND USE THEREOF
(FR) NOUVELLE SOLUTION DE MISE EN CULTURE D'UNE CULTURE PRIMAIRE DE NEURONES D'HIPPOCAMPES ET DE RATS, SON PROCÉDÉ DE PRÉPARATION ET SES APPLICATIONS
(ZH) 一种新生大鼠海马神经元原代培养的培养液及其制备方法和应用
Abstract:
(EN) Provided is a culture medium for the primary culture of hippocampus neurons of a neonatal rat. The culture medium is formed by adding 1,6-diphosphate fructose, fructose and nutritional supplements to a basic medium Neurobasal. Also provided is a method for the primary culture of hippocampus neurons of a rat using the abovementioned culture medium, comprising the steps of: (1) preparing the culture medium of hippocampus neurons; (2) sampling and digesting; (3) preparing a single cell suspension; (4) inoculating and culturing. Hippocampus neurons with good growing status and higher purity can be obtained using the abovementioned culture medium.
(FR) Fait l'objet de cette invention une solution de culture pour la mise en culture de neurones d'hippocampes et de rats. Cette solution de culture est un 1,6-diphosphate, un fructose et un additif nutritif ajoutés à une culture de base en un milieu Neurobasal. Fait aussi l'objet de cette invention un procédé mettant en oeuvre la mise en culture d'une culture primaire de neurones d'hippocampes et de rats comprenant les étapes suivantes : a) préparation d'une solution de culture de neurones d'hippocampes, b) sélection du matériel et leçons, c) préparation d'une suspension monocellulaire, d) inoculation et mise en culture. Cette solution de culture présente un bon état de croissance et les neurones d'hippocampes font preuve d'un niveau élevé de pureté.
(ZH) 提供了一种用于新生大鼠海马神经元原代培养的培养液。所述培养液是在基础培养液Neurobasal中加入1,6-二磷酸果糖、果糖和营养添加剂。还提供了使用上述培养液原代培养大鼠海马神经元的方法,包括步骤:(1)制备海马神经元培养液,(2)取材、消化,(3)制备单细胞悬浮液,(4)接种、培养。使用上述培养液能够获得生长状态良好,纯度较高的海马神经元。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)