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1. (WO2013110891) USE OF AN ACELLULAR EMPIRICAL CULTURE MEDIUM FOR THE GROWTH OF INTRACELLULAR BACTERIA
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2013/110891 International Application No.: PCT/FR2013/050137
Publication Date: 01.08.2013 International Filing Date: 23.01.2013
IPC:
C12N 1/20 (2006.01) ,C12Q 1/04 (2006.01) ,C12R 1/91 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
91
Cell lines
Applicants: FONDATION MEDITERRANEE INFECTION[FR/FR]; Faculte de Médecine 27, boulevard Jean Moulin F-13005 Marseille, FR
Inventors: RAOULT, Didier; FR
KOWALCZEWSKA, Malgorzata; FR
SINGH, Sudhir, Kumar; IN
Agent: DOMANGE, Maxime; Cabinet Beau de Loménie 232, avenue du Prado F-13295 Marseille Cedex 08, FR
Priority Data:
125079527.01.2012FR
Title (EN) USE OF AN ACELLULAR EMPIRICAL CULTURE MEDIUM FOR THE GROWTH OF INTRACELLULAR BACTERIA
(FR) UTILISATION D'UN MILIEU DE CULTURE EMPIRIQUE ACELLULAIRE POUR LA CROISSANCE DES BACTÉRIES INTRACELLULAIRES
Abstract:
(EN) The present invention relates to the use of an acellular empirical medium for culturing a given intracellular bacterium, able to be grown by cell culture in a given eukaryotic cell type, characterized in that said empirical medium comprises an aqueous suspension of a cell lysate consisting of a cytoplasmic juice comprising the endoplasmic content of said eukaryotic cells of a given type and the components and residues of said cell lysate which are as a dispersion or soluble in the cytosolic juice, preferably having sizes of less than 10 µm, more preferably less than 5 µm.
(FR) La présente invention concerne l'utilisation d'un milieu empirique acellulaire pour la culture d'une bactérie intracellulaire donnée, apte à être cultivée par culture cellulaire dans un type de cellule eucaryote donné, caractérisée en ce que ledit milieu empirique comprend une suspension aqueuse d'un lysat cellulaire constitué d'un jus cytoplasmique comprenant le contenu endoplasmique de dites cellules eucaryotes d'un type donné et des composants et résidus dudit lysat cellulaire en dispersion ou solubles dans le jus cytosolique, de préférence de tailles inférieure à 10 μm, de préférence encore inférieure à 5 μm.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: French (FR)
Filing Language: French (FR)