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74. (WO2013103901) DIELECTRIC SPECTROSCOPY METHODS AND APPARATUS

Pub. No.:    WO/2013/103901    International Application No.:    PCT/US2013/020397
Publication Date: Fri Jul 12 01:59:59 CEST 2013 International Filing Date: Sat Jan 05 00:59:59 CET 2013
IPC: G01N 27/22
Applicants: BEND RESEARCH, INC.
Inventors: DOWNEY, Brandon, J.
CARROLL, Adam, S.
GRAHAM, Lisa
BREIT, Jeffery, F.
Title: DIELECTRIC SPECTROSCOPY METHODS AND APPARATUS
Abstract:
Methods and apparatus are disclosed for correcting measurements us¬ ing calibration by applying a frequency-varying signal with a measur¬ ing device for performing dielectric spectroscopy (e.g., a permittivi¬ ty probe) to a population of living cells (e.g., contained in a bioreac- tor) and correcting measurement divergences using data acquired us¬ ing an alternate analytical method (e.g., trypan blue exclusion, hemo- cytometry or fluorescent flow cytometry). In one example, a method comprises receiving electrical property data for a first population of cells, the data obtained by applying a first frequency-varying signal to the population with the measuring device, receiving biological prop¬ erty data obtained using the alternate analytical technique, generat¬ ing at least one value representative of the frequency dependence of the electrical property data, and determining a relationship between the biological property data and the representative value. In some ex¬ amples, measurements of apoptosis are predicted using the electrical property data.