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1. WO2013098455 - METHOD AND KIT FOR DETERMINING THE ADMINISTRATION OF ESTRADIOL TO LIVESTOCK

Publication Number WO/2013/098455
Publication Date 04.07.2013
International Application No. PCT/ES2012/070914
International Filing Date 26.12.2012
IPC
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/74 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
74involving hormones
C07K 5/06 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
5Peptides having up to four amino acids in a fully defined sequence; Derivatives thereof
04containing only normal peptide links
06Dipeptides
CPC
C07K 5/06173
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
5Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
04containing only normal peptide links
06Dipeptides
06139with the first amino acid being heterocyclic
06173and Glp-amino acid; Derivatives thereof
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/743
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
74involving hormones ; or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
743Steroid hormones
Applicants
  • UNIVERSIDADE DE SANTIAGO DE COMPOSTELA [ES]/[ES]
Inventors
  • REGAL LÓPEZ, Patricia
  • FENTE SAMPAYO, Cristina
  • CEPEDA SÁEZ, Alberto
  • SEIJAS VÁZQUEZ, Julio Antonio
Agents
  • ACUÑA ÁLVAREZ, Laura
Priority Data
P20113212228.12.2011ES
Publication Language Spanish (es)
Filing Language Spanish (ES)
Designated States
Title
(EN) METHOD AND KIT FOR DETERMINING THE ADMINISTRATION OF ESTRADIOL TO LIVESTOCK
(ES) PROCEDIMIENTO Y KIT PARA DETERMINAR LA ADMINISTRACIÓN DE ESTRADIOL AL GANADO
(FR) PROCÉDÉ ET KIT PERMETTANT DE DÉTERMINER L'ADMINISTRATION D'ESTRADIOL À DU BÉTAIL
Abstract
(EN) The invention relates to a method and kit for determining the administration of estradiol to livestock. The invention discloses a method for determining the exogenous administration of 17β-estradiol to bovine animals, based on determining the D-pyroglutamyl-L-phenylalanine peptide in the serum of said animals. The presence of said peptide in greater concentrations than in the control animals indicates the exogenous administration of 17β-estradiol or an ester thereof. The invention also provides a kit which includes said peptide and enables the determination. The method can be carried out using various techniques, such as chromatographic separation combined with mass spectrometry, or immunoanalysis techniques such as ELISA.
(ES) Procedimiento y kit para determinar la administración de estradiol al ganado. Se divulga un procedimiento para determinar la administración exógena del 17β-estradiol a animales bovinos, basado en la determinación del péptido D-piroglutamil-L-fenilalanina en el suero de dichos animales. La presencia de dicho péptido en concentraciones superiores a las de los animales control indica la administración exógena de 17β- estradiol o un éster del mismo. También se proporciona un kit que comprende dicho péptido y que facilita la determinación. El procedimiento puede llevarse a cabo por diversas técnicas, tales como separación cromatográfica acoplada a espectrometría de masas, o técnicas de inmunoanálisis tales como ELISA.
(FR) Procédé et kit permettant de déterminer l'administration d'estradiol à du bétail. L'invention concerne un procédé pour déterminer l'administration exogène de 17β-estradiol à des bovins, sur la base de la détermination du peptide D-pyroglutamyl-L-phénylalanine dans le sérum de ces animaux. La présence de ce peptide à des concentrations supérieures à celles des animaux témoins indique l'administration exogène de 17β- estradiol ou d'un ester de celui-ci. L'invention concerne également un kit comprenant ce peptide et facilitant la détermination. Le procédé peut être mis en oeuvre par différentes techniques, telles que la séparation chromatographique couplée à la spectrométrie de masse, ou des techniques d'immunoanalyse, de type ELISA.
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