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1. (WO2013056546) MALDI-TOF MASS SPECTROMETRY DOUBLE INTERNAL STANDARDS AND QUANTITATIVE DETECTING METHOD THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2013/056546    International Application No.:    PCT/CN2012/074884
Publication Date: 25.04.2013 International Filing Date: 28.04.2012
IPC:
C12Q 1/68 (2006.01), C12N 15/11 (2006.01), G01N 27/62 (2006.01)
Applicants: MA, Qingwei [CN/CN]; (CN).
ZHANG, Haiyan [CN/CN]; (CN) (For US Only).
XING, Shuangyan [CN/CN]; (CN) (For US Only)
Inventors: MA, Qingwei; (CN).
ZHANG, Haiyan; (CN).
XING, Shuangyan; (CN)
Agent: UNITALEN ATTORNEYS AT LAW; 7th Floor, Scitech Place No.22, Jian Guo Men Wai Ave., Chao Yang District Beijing 100004 (CN)
Priority Data:
201110321059.7 21.10.2011 CN
Title (EN) MALDI-TOF MASS SPECTROMETRY DOUBLE INTERNAL STANDARDS AND QUANTITATIVE DETECTING METHOD THEREOF
(FR) MARQUEUR INTERNE DOUBLE MALDI-TOF POUR LA SPECTROMÉTRIE DE MASSE ET SON PROCÉDÉ DE DÉTECTION QUANTITATIVE
(ZH) MALDI-TOF质谱双内标及其定量检测方法
Abstract: front page image
(EN)The present invention provides an oligonucleotide pair used as double internal standards for mass spectrometry detection, containing LbC(ctcatcatgctgcccc) and LbT(ctcatcatgctgccct) for detecting an object to be tested of a low molecular weight, MbC(ccccatctaagcgcatcaaac) and MbT(ccccatctaagcgcatcaaat) for detecting an object to be tested of a medium molecular weight, and HbA(gttctcactcaattgtaaatgcacaa) and HbG(gttctcactcaattgtaaatgcacag) for detecting an object to be tested of a high molecular weight, which are used for establishing a double internal standard fitted curve for mass spectrometry detection.
(FR)L'invention porte sur des oligonucléotides utilisant des marqueurs internes doubles pour la détection par spectrométrie de masse et comprenant un LbC(ctcatcatgctgcccc) et un LbT(ctcatcatgctgccct) de détection d'un analyte à faible poids moléculaire, un MbC(ccccatctaagcgcatcaaac) et un MbT(ccccatctaagcgcatcaaat) de détection d'un analyte à poids moléculaire moyen, ainsi qu'un HbA(gttctcactcaattgtaaatgcacaa) et un HbG(gttctcactcaattgtaaatgcacag) de détection d'un analyte à poids moléculaire élevé servant à la construction d'une courbe de correspondance de marqueurs internes doubles pour la détection par spectrométrie de masse.
(ZH)本发明提供了一种用作质谱检测的双内标志物的寡核苷酸对,包含检测低分子量待检物的LbC(ctcatcatgctgcccc)和LbT(ctcatcatgctgccct)、检测中分子量待检物的MbC(ccccatctaagcgcatcaaac)和MbT(ccccatctaagcgcatcaaat)、检测高分子量待检物的HbA(gttctcactcaattgtaaatgcacaa)和HbG(gttctcactcaattgtaaatgcacag),用于建立质谱检测的双内标拟合曲线。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)