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1. WO2013051704 - GLUCOSE DEHYDROGENASE

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Publication Number WO/2013/051704
Publication Date 11.04.2013
International Application No. PCT/JP2012/075999
International Filing Date 05.10.2012
IPC
C12N 15/09 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12M 1/40 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
40Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
C12N 1/15 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
15modified by introduction of foreign genetic material
C12N 1/19 2006.1
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1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
16Yeasts; Culture media therefor
19modified by introduction of foreign genetic material
C12N 1/21 2006.1
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1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 5/10 2006.1
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5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
CPC
C12N 9/0006
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0006acting on CH-OH groups as donors (1.1)
C12Q 1/006
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
001Enzyme electrodes
005involving specific analytes or enzymes
006for glucose
C12Q 1/32
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
26involving oxidoreductase
32involving dehydrogenase
Applicants
  • 池田食研株式会社 IKEDA FOOD RESEARCH CO., LTD. [JP]/[JP]
Inventors
  • 本田　通済 HONDA, Michinari
  • 滝　さやか TAKI, Sayaka
  • 竹中　涼 TAKENAKA, Ryo
Agents
  • 特許業務法人アルガ特許事務所 THE PATENT CORPORATE BODY ARUGA PATENT OFFICE
Priority Data
2011-22074505.10.2011JP
2012-13611215.06.2012JP
Publication Language Japanese (ja)
Filing Language Japanese (JA)
Designated States
Title
(EN) GLUCOSE DEHYDROGENASE
(FR) GLUCOSE DÉSHYDROGÉNASE
(JA) グルコース脱水素酵素
Abstract
(EN) Provided are a glucose dehydrogenase originating in Botryotinia fuckeliana, a method for producing the same, and an assay method, a reagent composition and a biosensor each using the aforesaid enzyme. A biosensor for assaying glucose without being affected by dissolved oxygen, characterized by using a flavin-bound glucose dehydrogenase that comprises glycopolypeptide (e) or (f): (e) a glycopolypeptide comprising an amino acid sequence represented by SEQ ID NO:5; and (f) a glycopolypeptide comprising an amino acid sequence that is derived from the amino acid sequence represented by SEQ ID NO:5 by substitution, deletion or addition of one to several amino acids and having glucose dehydrogenase activity.
(FR) L'invention concerne une glucose déshydrogénase provenant de Botryotinia fuckeliana, un procédé de production de celle-ci, et une méthode de dosage, une composition de réactif et un biocapteur, utilisant chacun l'enzyme mentionnée ci-dessus. L'invention concerne un biocapteur pour le dosage du glucose sans être affecté par l'oxygène dissous, caractérisé en ce qu'il utilise une glucose déshydrogénase liée à une flavine qui comprend un glycopolypeptide (e) ou (f) : (e) un glycopolypeptide comprenant une séquence d'acides aminés représentée par SEQ ID No:5 ; et (f) un glycopolypeptide comprenant une séquence d'acides aminés qui est issue de la séquence d'acides aminés représentée par SEQ ID No:5 par substitution, délétion ou addition d'un à plusieurs acides aminés et ayant une activité glucose déshydrogénase.
(JA) 　ボトリオティニア・フケリアナ由来のグルコース脱水素酵素及びその製造方法、並びに該酵素を用いた測定方法、試薬組成物及びバイオセンサを提供するものである。　以下の（ｅ）又は（ｆ）の糖ポリペプチドからなるフラビン結合型グルコース脱水素酵素を使用することを特徴とする、溶存酸素の影響を受けないグルコース測定用バイオセンサ： （ｅ）配列番号５で示されるアミノ酸配列からなる糖ポリペプチド、 （ｆ）配列番号５で示されるアミノ酸配列において１～数個のアミノ酸が置換、欠失又は付加されたアミノ酸配列からなり、かつグルコース脱水素酵素活性を有する糖ポリペプチド。
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