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1. (WO2013048163) CONVENIENT AND EFFICIENT PURIFICATION METHOD FOR CHEMICALLY LABELED NUCLEIC ACIDS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2013/048163    International Application No.:    PCT/KR2012/007870
Publication Date: 04.04.2013 International Filing Date: 27.09.2012
IPC:
C12N 15/10 (2006.01), C12Q 1/68 (2006.01)
Applicants: SNU R&DB FOUNDATION [KR/KR]; 1 Gwanak-ro, Gwanak-gu, Seoul 151-742 (KR)
Inventors: KIM, Seong Keun; (KR).
KIM, Young Gyu; (KR).
HWANG, Ji Hee; (KR)
Agent: KOREANA PATENT FIRM; (Yeoksam-dong, Dong-kyung Bldg.) 11Fl. 116 Teheran-ro, Gangnam-gu Seoul 135-080 (KR)
Priority Data:
10-2011-0099061 29.09.2011 KR
10-2012-0020649 28.02.2012 KR
10-2012-0104438 20.09.2012 KR
Title (EN) CONVENIENT AND EFFICIENT PURIFICATION METHOD FOR CHEMICALLY LABELED NUCLEIC ACIDS
(FR) PROCÉDÉ DE PURIFICATION PRATIQUE ET EFFICACE D'ACIDES NUCLÉIQUES MARQUÉS CHIMIQUEMENT
Abstract: front page image
(EN)The present invention relates to a method for purifying chemically labeled nucleic acids. More particularly, the present invention relates to a convenient and efficient method for purifying labeled nucleic acids in a fast manner with high efficiency from a mixture of unreacted hydrophobic probes, unreacted nucleic acids and labeled nucleic acids. In particular, the present invention relates to a convenient method for purifying labeled nucleic acids in a fast manner with high efficiency from a mixture of unreacted hydrophobic chemical probes, unreacted nucleic acids and labeled nucleic acids, comprising the steps of reacting hydrophobic chemical probe and nucleic acids in an aqueous solution to prepare a reacted solution; adding organic solvent to the reacted solution and stirring it to obtain a mixed solution; and centrifuging the mixed solution and removing organic solvent phase.
(FR)Cette invention concerne un procédé de purification d'acides nucléiques marqués chimiquement, et plus particulièrement, un procédé de purification pratique et efficace d'acides nucléiques marqués chimiquement, rapide, à rendement élevé, à partir d'un mélange de sondes hydrophobes n'ayant pas réagi, d'acides nucléiques n'ayant pas réagi et d'acides nucléiques marqués, ledit procédé comprenant les étapes consistant à faire réagir la sonde chimique hydrophobe et les acides nucléiques dans une solution aqueuse pour préparer une solution réactive ; à ajouter un solvant organique à la solution réactive et à agiter pour obtenir une solution homogène ; à centrifuger la solution homogène et à éliminer la phase solvant organique.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)