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1. (WO2013046226) A NOVEL QUANTIFICATION METHOD FOR VACCINES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2013/046226    International Application No.:    PCT/IN2012/000541
Publication Date: 04.04.2013 International Filing Date: 06.08.2012
IPC:
A61K 39/09 (2006.01)
Applicants: SERUM INSTITUTE OF INDIA LTD. [IN/IN]; 212/2 Off Soli Poonawalla Road Hadapsar Maharashtra Pune 411028 (IN) (For All Designated States Except US).
KAPRE, Subhash Vinayak [IN/IN]; (IN) (For US Only).
CHHIKARA, Manoj Kumar [IN/IN]; (IN) (For US Only).
SONI, Dipen Jagdishbhai [IN/IN]; (IN) (For US Only)
Inventors: KAPRE, Subhash Vinayak; (IN).
CHHIKARA, Manoj Kumar; (IN).
SONI, Dipen Jagdishbhai; (IN)
Agent: WILSON, Neeti; B-41 Nizamuddin East New Delhi 110013 (IN)
Priority Data:
2215/MUM/2011 04.08.2011 IN
Title (EN) A NOVEL QUANTIFICATION METHOD FOR VACCINES
(FR) NOUVEAU PROCÉDÉ DE QUANTIFICATION POUR VACCINS
Abstract: front page image
(EN)To provide a novel and efficient method for estimation of free polysaccharides in multivalent polysaccharide protein conjugate vaccines, wherein anti-carrier protein antibody coupled beads are mixed with sample containing multivalent protein polysaccharide conjugates to enable the separation of conjugated and unconjugated polysaccharide followed by estimation of free polysaccharide content from supernatant by multiplex competitive inhibition assay. The instant method overcomes the limitations of HPSEC & Deoxycholate based methods for free polysaccharide estimation in multivalent polysaccharide protein conjugate vaccines. Additionally total polysaccharide content and percent adsorption of polysaccharides can also be measured by instant method.
(FR)La présente invention concerne un nouveau procédé efficace d'estimation de polysaccharides libres dans des vaccins polysaccharidiques multivalents associés à des protéines, des billes couplées à des anticorps anti-protéines porteuses étant mélangées à un échantillon contenant des conjugués polysaccharidiques multivalents associés à des protéines pour permettre de séparer les polysaccharides conjugués et ceux non-conjugués et d'estimer ensuite la teneur en polysaccharides libres dans le surnageant en utilisant un dosage par inhibition compétitive multiplex. Le procédé de l'invention surmonte les limitations des procédés de HPSEC et ceux basés sur le désoxycholate dans l'estimation des polysaccharides libres présents dans les vaccins polysaccharidiques multivalents associés à des protéines. De plus, la teneur totale en polysaccharides et le pourcentage d'adsorption de polysaccharides peuvent également être mesurés par le procédé de l'invention.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)