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1. WO2013014537 - VIRUS-BASED VECTOR COMPOSITIONS USEFUL FOR TRANSDUCING EUKARYOTIC CELLS

Publication Number WO/2013/014537
Publication Date 31.01.2013
International Application No. PCT/IB2012/001807
International Filing Date 26.07.2012
IPC
C12N 7/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
02Recovery or purification
CPC
C12N 2740/15051
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
15011Lentivirus, not HIV, e.g. FIV, SIV
15051Methods of production or purification of viral material
C12N 7/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
02Recovery or purification
Applicants
  • VECTALYS [FR]/[FR] (AllExceptUS)
  • BOUILLE, Pascale [FR]/[FR] (UsOnly)
  • VERGNAULT, Hélène [FR]/[FR] (UsOnly)
  • GAYON, Régis [FR]/[FR] (UsOnly)
  • MOAL, Yohann [FR]/[FR] (UsOnly)
Inventors
  • BOUILLE, Pascale
  • VERGNAULT, Hélène
  • GAYON, Régis
  • MOAL, Yohann
Agents
  • SANTARELLI
Priority Data
61/512,28927.07.2011US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) VIRUS-BASED VECTOR COMPOSITIONS USEFUL FOR TRANSDUCING EUKARYOTIC CELLS
(FR) COMPOSITIONS DE VECTEUR À BASE DE VIRUS UTILES POUR LA TRANSDUCTION DE CELLULES EUCARYOTES
Abstract
(EN)
The present invention provides viral vector compositions of high titer and purity, as well as methods for production of said compositions. The methods of the invention incorporate multiple features, such as production of viral vector particles in serum free media and multiple harvesting steps following transduction of the producer cell which provides for enhanced production of said viral vectors. The viral vector compositions of the invention, by virtue of their high titer and purity, minimize the deleterious phenotypic changes that typically occur following transduction of target cells, such as loss of a sub-population of transduced cells, and effects on proliferation, differentiation, reprogramming or functionality of transduced cells.
(FR)
La présente invention concerne des compositions de vecteurs viraux au titre et à la pureté élevés, ainsi que des procédés de production desdites compositions. Les procédés de l'invention comprennent de multiples caractéristiques, telles que la production de particules de vecteurs viraux dans des milieux exempts de sérum et de multiples étapes de récolte suivant la transduction de la cellule productrice qui permettent une production améliorée desdits vecteurs viraux. Les compositions de vecteurs viraux de l'invention, grâce à leur titre et leur pureté élevées, rendent minimales les modifications phénotypiques délétères qui typiquement ont lieu à la suite de la transduction des cellules cibles, telles que la perte d'une sous-population de cellules transduites, et agissent sur la prolifération, la différentiation, la reprogrammation et la fonctionnalité des cellules transduites.
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