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1. WO2013013687 - APPARATUS AND METHOD FOR A LYSIS OF A SAMPLE, IN PARTICULAR FOR AN AUTOMATED AND/OR CONTROLLED LYSIS OF A SAMPLE

Publication Number WO/2013/013687
Publication Date 31.01.2013
International Application No. PCT/EP2011/003770
International Filing Date 27.07.2011
IPC
G01N 1/28 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
G01N 1/38 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
38Diluting, dispersing or mixing samples
G01N 1/44 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
44Sample treatment involving radiation, e.g. heat
CPC
C12M 47/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
47Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
C12N 1/066
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
06Lysis of microorganisms
066by physical methods
G01N 1/286
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
286involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
G01N 1/38
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
38Diluting, dispersing or mixing samples
G01N 1/44
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
44Sample treatment involving radiation, e.g. heat
G01N 2035/00504
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
00465Separating and mixing arrangements
00495Centrifuges
00504combined with carousels
Applicants
  • CURETIS AG [DE]/[DE] (AllExceptUS)
  • BOOS, Andreas [DE]/[DE] (UsOnly)
  • LÜDKE, Gerd [DE]/[DE] (UsOnly)
  • BACHER, Johannes [DE]/[DE] (UsOnly)
Inventors
  • BOOS, Andreas
  • LÜDKE, Gerd
  • BACHER, Johannes
Agents
  • SCHOLLWECK, Susanne
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) APPARATUS AND METHOD FOR A LYSIS OF A SAMPLE, IN PARTICULAR FOR AN AUTOMATED AND/OR CONTROLLED LYSIS OF A SAMPLE
(FR) APPAREIL ET PROCÉDÉ DE LYSE D'UN ÉCHANTILLON, EN PARTICULIER POUR LA LYSE AUTOMATISÉE ET / OU RÉGULÉE D'UN ÉCHANTILLON
Abstract
(EN)
The present invention provides an apparatus and a method for a lysis procedure, in particular for an automated and/or controlled lysis procedure of a sample, in particular a biological sample. The apparatus comprises at least one rotation disc (31), at least one vial holder (90) which is configured to receive a vial (100), wherein the vial holder (90) is arranged on the disc (31), at least one driving device (20) which is configured to rotate the disc (31) and the vial holder (90), at least one heating device (60) which is configured to heat the sample to a determined incubation temperature, and - at least one control device (70) which is configured to control the driving device (20) and/or the heating device (60) by means of a timing and/or step control, and/or - at least one transmitting device (80) for inductive coupling for energy and signal transmission, which is configured to transmit the energy for heating to the heating device (60), and/or - wherein the driving device (20) is configured to rotate the disc (31) in a first direction (A1) and/or with a first speed, and to rotate the vial holder (90) in a second direction (A3) and/or with a second speed. The apparatus and the method are adapted for an (automated) lysis procedure, wherein the lysis can be carried out in a safe, efficient and effective manner.
(FR)
La présente invention concerne un appareil et un procédé destinés à une procédure de lyse, en particulier destinés à une procédure automatisée et / ou régulée de lyse d'un échantillon, en particulier d'un échantillon biologique. L'appareil comporte au moins un disque (31) de rotation, au moins un porte-fiole (90) configuré pour recevoir une fiole (100), le porte-fiole (90) étant placé sur le disque (31), au moins un dispositif (20) d'entraînement configuré pour faire tourner le disque (31) et le porte-fiole (90), au moins un dispositif (60) de chauffage configuré pour chauffer l'échantillon jusqu'à une température d'incubation déterminé, and au moins un dispositif (70) de régulation configuré pour commander le dispositif (20) d'entraînement et / ou le dispositif (60) de chauffage au moyen d'une temporisation et / ou d'une régulation par échelons, et / ou au moins un dispositif émetteur (80) destiné à un couplage inductif pour une émission d'énergie et de signaux, et configuré pour émettre l'énergie servant au chauffage vers le dispositif (60) de chauffage, et / ou le dispositif (20) d'entraînement étant configuré pour faire tourner le disque (31) dans un premier sens (A1) et / ou à une première vitesse, et pour faire tourner le porte-fiole (90) dans un deuxième sens (A3) et / ou à une deuxième vitesse. L'appareil et le procédé sont conçus pour une procédure de lyse (automatisée), la lyse pouvant être réalisée de manière sûre, efficiente et efficace.
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