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1. WO2013013229 - INSTRUMENT AND METHOD FOR OPTICAL PARTICLE SENSING

Publication Number WO/2013/013229
Publication Date 24.01.2013
International Application No. PCT/US2012/047766
International Filing Date 20.07.2012
IPC
G01N 15/02 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
02Investigating particle size or size distribution
G01N 33/48 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
CPC
G01N 15/0211
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
02Investigating particle size or size distribution
0205by optical means, e.g. by light scattering, diffraction, holography or imaging
0211Investigating a scatter or diffraction pattern
G01N 15/1434
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1434using an analyser being characterised by its optical arrangement
G01N 15/1459
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1456without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
1459the analysis being performed on a sample stream
G01N 2015/0065
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
0065biological, e.g. blood
G01N 2015/0073
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
0065biological, e.g. blood
0073Red blood cells
G01N 2015/008
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
0065biological, e.g. blood
008White cells
Applicants
  • INVITROX, INC. [US]/[US] (AllExceptUS)
  • GABRIEL, Don [US]/[US] (UsOnly)
Inventors
  • GABRIEL, Don
Agents
  • BRADIN, David
Priority Data
61/510,44721.07.2011US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) INSTRUMENT AND METHOD FOR OPTICAL PARTICLE SENSING
(FR) INSTRUMENT ET PROCÉDÉ DE DÉTECTION DE PARTICULES OPTIQUES
Abstract
(EN)
Devices for detecting particle sizes and distributions using focused light scattering techniques, by passing a sample through a focused beam of light, are disclosed. In one embodiment, the devices include one or more lasers, whose light is focused into a narrow beam and into a flow cell, and dispersions are passed through the flow cell using hydrodynamic sample injection. In another embodiment, a plurality of lasers is used, optionally with hydrodynamic sample injection. Particles pass through and scatter the light. The scattered light is then detected using scatter and extinction detectors, and, optionally, fluorescence detectors, and the number and size of the particles is determined. Particles in the size range of 0.1 to 10 μιη can be measured. Using the device, significantly smaller particles can be detected than if techniques such as EQELS, flow cytometry, and other conventional devices for measuring biological particles.
(FR)
L'invention concerne des dispositifs de détection de tailles et de répartitions de particules utilisant des techniques de dispersion de lumière concentrée, en faisant passer un échantillon à travers un faisceau concentré de lumière. Dans un mode de réalisation, les dispositifs comprennent un ou plusieurs lasers, dont la lumière est concentrée en un faisceau étroit et entre dans une cellule d'écoulement, et des dispersions sont envoyées à travers la cellule d'écoulement par injection hydrodynamique d'échantillons. Dans un autre mode de réalisation, une pluralité de lasers est utilisée, éventuellement avec une injection hydrodynamique d'échantillons. Des particules passent à travers la lumière et la dispersent. La lumière dispersée est alors détectée à l'aide de détecteurs de dispersion et d'extinction et, éventuellement, de détecteurs de fluorescence, et le nombre et la taille des particules sont déterminés. Il est possible de mesurer des particules dans une plage de tailles allant de 0,1 à 10 μm. A l'aide du dispositif, il est possible de détecter des particules nettement plus petites que si des techniques comme EQELS, la cytométrie de flux et d'autres dispositifs conventionnels sont utilisés pour mesurer des particules biologiques.
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