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1. WO2013009739 - SOLUBLE TREPONEMA PALLIDUM PROTEIN TP0453,TP0453-TP0326 FUSION PROTEIN, AND USE IN SYPHILIS DIAGNOSIS

Publication Number WO/2013/009739
Publication Date 17.01.2013
International Application No. PCT/US2012/046040
International Filing Date 10.07.2012
IPC
C12N 15/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
CPC
C07K 14/20
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
20from Spirochaetales (O), e.g. Treponema, Leptospira
G01N 2333/20
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
195from bacteria
20from Spirochaetales (O), e.g. Treponema, Leptospira
G01N 2469/20
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2469Immunoassays for the detection of microorganisms
20Detection of antibodies in sample from host which are directed against antigens from microorganisms
G01N 33/56911
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
56911Bacteria
G01N 33/571
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
571for venereal disease, e.g. syphilis, gonorrhoea
Applicants
  • UVIC INDUSTRY PARTNERSHIPS INC. [CA]/[CA] (AllExceptUS)
  • CAMERON, Caroline E. [CA]/[CA] (UsOnly)
  • BOULANGER, Martin J. [CA]/[CA] (UsOnly)
  • SMITH, Brenden C. [US]/[CA] (UsOnly)
Inventors
  • CAMERON, Caroline E.
  • BOULANGER, Martin J.
  • SMITH, Brenden C.
Agents
  • RYBAK, Sheree Lynn
Priority Data
61/506,35211.07.2011US
61/602,15123.02.2012US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SOLUBLE TREPONEMA PALLIDUM PROTEIN TP0453,TP0453-TP0326 FUSION PROTEIN, AND USE IN SYPHILIS DIAGNOSIS
(FR) PROTÉINE TP0453 DE TREPONEMA PALLIDUM SOLUBLE, PROTÉINE HYBRIDE TP0453-TP0326 ET LEUR UTILISATION DANS LE DIAGNOSTIC DE LA SYPHILIS
Abstract
(EN)
The present application provides methods of producing soluble Treponema pallidum protein Tp0453 (such as the fragment shown in SEQ ID NO: 3). For example, the protein can be expressed from the pET28a vector in a cell and the resulting soluble Tp0453 protein isolated from the cell. Also provided are the isolated soluble Tp0453 protein, as well as Tp0453-Tp0326 chimeric proteins (e.g., SEQ ID NO: 11), and methods of using the proteins to detect antibodies specific for T. pallidum subsp. pallidum, for example to diagnose syphilis. Devices and kits that incorporate Tp0453 protein and Tp0453-Tp0326 chimeric proteins, such as lateral flow devices, are also disclosed. Kits that include the soluble Tp0453 protein and kits that include solid substrates containing the soluble Tp0453 protein are also provided.
(FR)
La présente invention porte sur des procédés d'obtention de la protéine Tp0453 de Treponema pallidum soluble (telle que le fragment représenté dans SEQ ID NO : 3). Par exemple, la protéine peut être exprimée par le vecteur pET28a dans une cellule et la protéine Tp0453 soluble ainsi obtenue peut être isolée de la cellule. L'invention porte également sur la protéine Tp0453 soluble isolée, ainsi que sur des protéines chimériques Tp0453-Tp0326 (par exemple SEQ ID NO : 11) et sur des procédés d'utilisation des protéines pour détecter des anticorps spécifiques pour T. pallidum sous-espèce pallidum, par exemple pour diagnostiquer la syphilis. L'invention porte également sur des dispositifs et sur des trousses qui comportent la protéine Tp0453 et les protéines chimériques Tp0453-Tp0326, tels que des dispositifs à débit latéral. L'invention porte également sur des trousses qui comprennent la protéine Tp0453 soluble et sur des trousses qui comprennent des substrats solides contenant la protéine Tp0453 soluble.
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