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1. (WO2012172129) OPTIMISED METHOD FOR OBTAINING ANGIOTENSIN CONVERTING ENZYME INHIBITORY PEPTIDES, INHIBITORY PEPTIDES AND FOOD CONTAINING SAME
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2012/172129 International Application No.: PCT/ES2011/070551
Publication Date: 20.12.2012 International Filing Date: 27.07.2011
IPC:
C12P 21/06 (2006.01) ,A23J 3/34 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
06
produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
A HUMAN NECESSITIES
23
FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
J
PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
3
Working-up of proteins for foodstuffs
30
by hydrolysis
32
using chemical agents
34
using enzymes
Applicants:
QUEIZUAR, S.L. [ES/ES]; A Silva - Bama Touro E-15822 A Coruña, ES (AllExceptUS)
PEREIRA RAMOS, Benigno [ES/ES]; ES (UsOnly)
PEREIRA RODRIGUEZ, Angel [ES/ES]; ES (UsOnly)
ESTEVEZ TELLE, Natalia [ES/ES]; ES (UsOnly)
FUCIÑOS GONZALEZ, Juan Pablo [ES/ES]; ES (UsOnly)
RUA RODRIGUEZ, Maria Luisa [ES/ES]; ES (UsOnly)
PASTRANA CASTRO, Lorenzo [ES/ES]; ES (UsOnly)
Inventors:
PEREIRA RAMOS, Benigno; ES
PEREIRA RODRIGUEZ, Angel; ES
ESTEVEZ TELLE, Natalia; ES
FUCIÑOS GONZALEZ, Juan Pablo; ES
RUA RODRIGUEZ, Maria Luisa; ES
PASTRANA CASTRO, Lorenzo; ES
Agent:
CARVAJAL Y URQUIJO, Isabel; Goya, 11 Clarke, Modet & Co E-28001 Madrid, ES
Priority Data:
20113100115.06.2011ES
Title (EN) OPTIMISED METHOD FOR OBTAINING ANGIOTENSIN CONVERTING ENZYME INHIBITORY PEPTIDES, INHIBITORY PEPTIDES AND FOOD CONTAINING SAME
(ES) PROCEDIMIENTO OPTIMIZADO PARA LA OBTENCION DE PEPTIDOS INHIBIDORES DE LA ENZIMA CONVERTIDORA DE ANGIOTENSINA; PEPTIDOS INHIBIDORES Y ALIMENTO QUE LOS COMPRENDE
(FR) PROCÉDÉ OPTIMISÉ POUR L'OBTENTION DE PEPTIDES INHIBITEURS DE L'ENZYME DE CONVERSION DE L'ANGIOTENSINE, PEPTIDES INHIBITEURS ET ALIMENT LES COMPRENANT
Abstract:
(EN) The invention relates to an optimised method for obtaining ACE-inhibitory peptides, comprising the following steps: ultrafiltration of whey in order to obtain a serum concentrate, enzymatic hydrolysis, ultrafiltration of hydrolysates and separation of the inhibitory peptides. The method also comprises the selection and addition of a mixture formed by a suitable ratio and quantity of two microbial proteases having a complementary action to the serum concentrate, for the hydrolysis of same, in order to obtain peptides with an ACE inhibition percentage greater than 70% and an IC50 index of between 30 and 100 μg/ml.
(ES) Procedimiento optimizado para obtener péptidos inhibidores de la actividad de la ECA, en el que están presentes las etapas de ultrafiltración de suero lácteo para obtener un concentrado de suero, hidrólisis enzimática, ultrafiltración de hidrolizados y separación de ios péptidos inhibidores, que comprende una selección y adición de una mezcla adecuada en proporción y cantidad de dos proteasas de origen microbiano y acción complementaria a dicho concentrado de suero, para la hidrólisis de éste, con el fin de obtener péptidos con un porcentaje de inhibición de la ECA superior ai 70% y un índice iC50 de 30 a 100 Mg/ml.
(FR) L'invention concerne un procédé optimisé pour obtenir des peptides inhibiteurs de l'activité de l'ECA, comprenant les étapes suivantes: ultrafiltration du lactosérum pour obtenir un concentré de sérum, hydrolyse enzymatique, ultrafiltration d'hydrolysats et séparation des peptides inhibiteurs, avec sélection et addition d'un mélange approprié en proportion et en quantité de deux protéases d'origine microbienne et à action complémentaire audit concentré de sérum, pour l'hydrolyse de celui-ci, en vue d'obtenir des peptides présentant un pourcentage d'inhibition de l'ECA supérieur à 70% et un indice IC50 de 30 à 100 μg/ml.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Spanish (ES)
Filing Language: Spanish (ES)
Also published as:
US20120322745EP2728011DK2728011ES2657366PT2728011