WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
maximize
Machine translation
1. (WO2012154201) ORTHOGONAL AMPLIFICATION AND ASSEMBLY OF NUCLEIC ACID SEQUENCES
Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

What is claimed is:

1. A microarray comprising at least 5,000 different oligonucleotide sequences attached thereto,

wherein each oligonucleotide sequence is a member of one of a plurality of oligonucleotide sets, and each oligonucleotide set is specific for a nucleic acid sequence of interest,

wherein each oligonucleotide sequence that is a member of a particular oligonucleotide set includes a pair of orthogonal primer binding sites having a sequence that is unique to said oligonucleotide set, and

wherein the nucleic acid sequence of interest is at least 500 nucleotides in length.

2. The microarray of claim 1, wherein at least 50 oligonucleotide sets are provided, and

wherein each set is specific for a unique nucleic acid sequence of interest.

3. The microarray of claim 1, wherein at least 100 oligonucleotide sets are provided, and

wherein each set is specific for a unique nucleic acid sequence of interest.

4. The microarray of claim 1 , wherein the oligonucleotide sequence of interest is at least

1,000 nucleotides in length.

5. The microarray of claim 1, wherein the oligonucleotide sequence of interest is at least

2,500 nucleotides in length.

6. The microarray of claim 1, wherein the oligonucleotide sequence of interest is at least

5,000 nucleotides in length.

7. The microarray of claim 1, wherein the nucleic acid sequence of interest is a DNA sequence.

8. The microarray of claim 7, wherein the DNA sequence is selected from the group consisting of a regulatory element, a gene, a pathway and a genome.

9. The microarray of claim 1, comprising at least 10,000 different oligonucleotide

sequences attached thereto.

10. The microarray of claim 1, wherein an oligonucleotide set is specific for a single

nucleic acid sequence of interest.

11. A microarray comprising at least 10,000 different oligonucleotide sequences attached thereto,

wherein each oligonucleotide sequence is a member of one of at least 50 oligonucleotide sets, and each oligonucleotide set is specific for a nucleic acid sequence of interest,

wherein each oligonucleotide sequence that is a member of a particular oligonucleotide set includes a pair of orthogonal primer binding sites having a sequence that is unique to said oligonucleotide set, and

wherein each nucleic acid sequence of interest is at least 2,500 nucleotides in length.

12. A method of synthesizing a nucleic acid sequence of interest comprising the steps of: providing at least 5,000 different oligonucleotide sequences, wherein each oligonucleotide sequence is a member of one of a plurality of oligonucleotide sets, and each oligonucleotide set is specific for a nucleic acid sequences of interest, and wherein each oligonucleotide sequence includes a pair of orthogonal primer binding sites having a sequence that is unique to a single oligonucleotide set;

amplifying an oligonucleotide set using orthogonal primers that hybridize to the orthogonal primer binding sites unique to the set;

removing the orthogonal primer binding sites from the amplified oligonucleotide set; and

assembling the amplified oligonucleotide set into a nucleic acid sequence of interest that is at least 500 nucleotides in length.

13. The method of claim 12, wherein the nucleic acid sequence of interest is at least 1,000 nucleotides in length.

14. The method of claim 12, wherein the nucleic acid sequence of interest is at least 2,500 nucleotides in length.

15. The method of claim 12, wherein the nucleic acid sequence of interest is at least 5,000 nucleotides in length.

16. The method of claim 12, wherein the nucleic acid sequence of interest is a DNA sequence.

17. The method of claim 16, wherein the DNA sequence is selected from the group consisting of a regulatory element, a gene, a pathway and a genome.

18. The method of claim 12, wherein 50 oligonucleotide sets are provided, and wherein each set is specific for a unique nucleic acid sequence of interest.

19. The method of claim 12, wherein 100 oligonucleotide sets are provided, and wherein each set is specific for a unique nucleic acid sequence of interest.

20. The method of claim 12, wherein 500 oligonucleotide sets are provided, and wherein each set is specific for a unique nucleic acid sequence of interest.

21. The method of claim 12, wherein 750 oligonucleotide sets are provided, and wherein each set is specific for a unique nucleic acid sequence of interest.

22. The method of claim 12, wherein 1,000 oligonucleotide sets are provided, and wherein each set is specific for a unique nucleic acid sequence of interest.

23. The method of claim 12, wherein the 5,000 different oligonucleotide sequences are provided on a microarray.

24. The method of claim 23, wherein the 5,000 different oligonucleotide sequences are removed from the microarray prior to the step of amplifying.