WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2012129547) FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2012/129547 International Application No.: PCT/US2012/030467
Publication Date: 27.09.2012 International Filing Date: 23.03.2012
IPC:
C12Q 1/68 (2006.01)
Applicants: VOROBIEV, Alexei[RU/US]; US (UsOnly)
LUKHTANOV, Eugeny, A.[US/US]; US (UsOnly)
SCARR, Noah[US/US]; US (UsOnly)
ELITECH HOLDING B.V.[NL/NL]; Van Rensselaerweg 4 NL-6956 AV Spankeren, NL (AllExceptUS)
Inventors: VOROBIEV, Alexei; US
LUKHTANOV, Eugeny, A.; US
SCARR, Noah; US
Agent: BORRELLI, Sara K.; Jackson Walker L.L.P. 901 Main Street, Suite 6000 Dallas, TX 75202, US
Priority Data:
61/466,75523.03.2011US
Title (EN) FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
(FR) ANALOGUES DE 3-ALCYNYL PYRAZOLOPYRIMIDINES FONCTIONNALISÉES COMME BASES UNIVERSELLES ET PROCÉDÉS D'UTILISATION
Abstract: front page image
(EN) 3-aIkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
(FR) L'invention concerne des analogues de 3-alcynyl inosine et leurs utilisations comme bases universelles. Les analogues d'inosine peuvent être incorporés dans des amorces et sondes d'acide nucléique. Lesdits analogues ne déstabilisent pas de façon significative des duplex d'acide nucléique. Par conséquent, les nouvelles amorces et sondes d'acide nucléique incorporant les analogues d'inosine peuvent être utilisées dans une diversité de procédés. Les analogues fonctionnent remarquablement bien, de façon inattendue, en tant que bases universelles. Non seulement ils stabilisent des duplex sensiblement plus que l'hypoxanthine contre A, C, T et G, mais encore ils sont reconnus dans des amorces par les polymérases, permettant une amplification efficace.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)