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1. (WO2012125781) RAPID DETECTION OF PATHOGENS USING PAPER DEVICES
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2012/125781 International Application No.: PCT/US2012/029156
Publication Date: 20.09.2012 International Filing Date: 15.03.2012
IPC:
G01N 33/52 (2006.01) ,G01N 21/78 (2006.01) ,C12Q 1/02 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52
Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
75
Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77
by observing the effect on a chemical indicator
78
producing a change of colour
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
Applicants: HENRY, Charles S.[US/US]; US (UsOnly)
GOODRIDGE, Lawrence D.[CA/US]; US (UsOnly)
JOKERST, Jana Catherine[US/US]; US (UsOnly)
COLORADO STATE UNIVERSITY RESEARCH FOUNDATION[US/US]; P.O. Box 483 Fort Collins, Colorado 80522, US (AllExceptUS)
Inventors: HENRY, Charles S.; US
GOODRIDGE, Lawrence D.; US
JOKERST, Jana Catherine; US
Agent: MCGAW, Michael M.; 180 Pine Avenue North Oldsmar, Florida 34677, US
Priority Data:
61/453,04215.03.2011US
Title (EN) RAPID DETECTION OF PATHOGENS USING PAPER DEVICES
(FR) DÉTECTION RAPIDE DE PATHOGÈNES UTILISANT DES DISPOSITIFS EN PAPIER
Abstract:
(EN) A kit for the rapid detection of pathogens in food supplies. The kit includes a microspot device and one or more indicator reagents to be applied to a well of the microspot device. The employed indicator reagent produces a detectable change upon contact with a pathogen of interest. The microspot device is fabricated from a porous membrane, such as filter paper. A substantially continuous boundary composed of a low melting temperature solid is deposited within the porous membrane extending from the top of the membrane to the bottom of the membrane and defines the peripheral sides of the well. Additionally, a barrier is applied to the bottom of the membrane, thus defining the bottom of the well. The kit can further include growth media for enriching the pathogenic bacteria and instructions for use of the kit employing the microspot device and the one or more indicator reagents.
(FR) L'invention concerne un kit pour la détection rapide de pathogènes dans des produits alimentaires. Le kit comprend un dispositif à microspots et un ou plusieurs réactifs indicateurs à appliquer à un puits du dispositif à microspots. Le réactif indicateur employé produit un changement détectable lors du contact avec un pathogène d'intérêt. Le dispositif à microspots est fabriqué à partir d'une membrane poreuse, telle qu'un papier de filtration. Une limite essentiellement continue composée d'un solide à température de fusion basse est déposée dans la membrane poreuse, s'étendant du haut de la membrane jusqu'en bas de la membrane, et définit les côtés périphériques du puits. Une barrière est également appliquée au fond de la membrane, définissant ainsi le fond du puits. Le kit peut également comprendre un milieu de croissance pour enrichir les bactéries pathogènes et des instructions d'utilisation du kit utilisant le dispositif à microspots et le ou les réactifs indicateurs.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)