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1. (WO2012112714) IMPROVED METHODS TO DETECT AND QUANTIFY RNA
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/112714    International Application No.:    PCT/US2012/025299
Publication Date: 23.08.2012 International Filing Date: 15.02.2012
IPC:
C12Q 1/68 (2006.01)
Applicants: INSTITUTE FOR SYSTEMS BIOLOGY [US/US]; 401 Terry Avenue North Seattle, WA 98109-5234 (US) (For All Designated States Except US).
WANG, Kai [US/US]; (US) (For US Only).
ZHANG, Shile [US/US]; (US) (For US Only).
GALAS, David [US/US]; (US) (For US Only)
Inventors: WANG, Kai; (US).
ZHANG, Shile; (US).
GALAS, David; (US)
Agent: MURASHIGE, Kate H.; MORRISON & FOERSTER LLP 12531 High Bluff Drive, Suite 100 San Diego, CA 92130-2040 (US)
Priority Data:
61/443,113 15.02.2011 US
Title (EN) IMPROVED METHODS TO DETECT AND QUANTIFY RNA
(FR) PROCÉDÉS AMÉLIORÉS POUR DÉTECTER ET QUANTIFIER L'ARN
Abstract: front page image
(EN)Improved methods to quantitate RNA in biological or other analytical samples employ extended RNAs containing adaptors at the 5' end and polyA sequences coupled to a tag at the 3 end. The invention method is particularly useful in quantitating microRNAs as primers can be used that need not complement the non-conserved 3' ends of these molecules.
(FR)La présente invention concerne des procédés améliorés pour quantifier l'ARN dans des échantillons biologiques ou analytiques qui utilisent des ARN étendus contenant des adaptateurs à l'extrémité 5' et des séquences polyA couplées à une étiquette à l'extrémité 3'. Le procédé de l'invention est particulièrement utile dans la quantification de microARN étant donné que des amorces peuvent être utilisées qui ne sont pas complémentaires des extrémités 3' non conservées de ces molécules.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)