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1. (WO2012110899) METHOD FOR LOCALIZED IN SITU DETECTION OF MRNA
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/110899    International Application No.:    PCT/IB2012/000995
Publication Date: 23.08.2012 International Filing Date: 15.02.2012
IPC:
C12Q 1/68 (2006.01)
Applicants: BERNITZ, Mats, Nilsson [SE/SE]; (SE).
LARSSON, Chatarina [SE/SE]; (SE).
GRUNDBERG, Ida [SE/SE]; (SE)
Inventors: BERNITZ, Mats, Nilsson; (SE).
LARSSON, Chatarina; (SE).
GRUNDBERG, Ida; (SE)
Agent: SHISHIMA, Gina, N.; Fulbright & Jaworski L.L.P. 98 San Jacinto Boulevard Suite 1100 Austin, TX 78701 (US)
Priority Data:
61/473,662 08.04.2011 US
61/442,921 15.02.2011 US
Title (EN) METHOD FOR LOCALIZED IN SITU DETECTION OF MRNA
(FR) PROCÉDÉ DE DÉTECTION D'ARNM IN SITU
Abstract: front page image
(EN)The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.
(FR)L'invention concerne la détection d'ARN dans un échantillon cellulaire. L'invention concerne en particulier la détection d'ARN in situ. Le procédé est basé sur la conversion d'ARN en ADN complémentaire avant le ciblage d'ADNc au moyen d'une ou de plusieurs sondes cadenas. L'hybridation de la ou des sondes cadenas est basée sur la séquence nucléotidique de l'ADNc qui est dérivée de la séquence nucléotidique correspondante de l'ARN cible. Une amplification par cercle roulant de la sonde cadenas subséquemment circularisée génère un produit de cercle roulant qui peut être détecté. De manière avantageuse, ceci permet de détecter l'ARN in situ.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)