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1. (WO2012109947) ULTRASOUND-ASSISTED POLLEN MEDIATED PLANT TRANSGENIC METHOD
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/109947    International Application No.:    PCT/CN2012/000030
Publication Date: 23.08.2012 International Filing Date: 09.01.2012
IPC:
C12N 15/82 (2006.01), A01H 1/00 (2006.01)
Applicants: BIOTECHNOLOGY RESEARCH CENTER, SHANXI ACADEMY OF AGRICULTURAL SCIENCES [CN/CN]; No.59 South Wucheng Road Taiyuan, Shanxi 030031 (CN) (For All Designated States Except US).
SUN, Yi [CN/CN]; (CN) (For US Only).
CUI, Guimei [CN/CN]; (CN) (For US Only).
HAO, Yaoshan [CN/CN]; (CN) (For US Only).
DU, Jianzhong [CN/CN]; (CN) (For US Only).
WANG, Yixue [CN/CN]; (CN) (For US Only).
YANG, Liyan [CN/CN]; (CN) (For US Only).
ZHANG, Hongmei [CN/CN]; (CN) (For US Only).
ZHANG, Lijun [CN/CN]; (CN) (For US Only).
WANG, Ming [CN/CN]; (CN) (For US Only).
ZHANG, Tingting [CN/CN]; (CN) (For US Only)
Inventors: SUN, Yi; (CN).
CUI, Guimei; (CN).
HAO, Yaoshan; (CN).
DU, Jianzhong; (CN).
WANG, Yixue; (CN).
YANG, Liyan; (CN).
ZHANG, Hongmei; (CN).
ZHANG, Lijun; (CN).
WANG, Ming; (CN).
ZHANG, Tingting; (CN)
Agent: TAIYUAN JINKE INTELLECTUAL PROPERTY ATTORNEY LAW OFFICE (SPECIAL GENERAL PARTNERSHIP); Room 1610, No.72 Ming ding Tower Taoyuan North Road Taiyuan, Shanxi 030002 (CN)
Priority Data:
201110041484.0 18.02.2011 CN
Title (EN) ULTRASOUND-ASSISTED POLLEN MEDIATED PLANT TRANSGENIC METHOD
(FR) PROCÉDÉ TRANSGÉNIQUE POUR PLANTE VÉHICULÉ PAR POLLEN ASSISTÉ PAR ULTRASONS
(ZH) 超声波辅助花粉介导植物转基因方法
Abstract: front page image
(EN)Disclosed is a plant transgenic method, which comprises using the Agrobacterium Ti plasmid, Escherichia coli plasmid, or other DNA vectors carrying an exogenous gene fragment as the gene donor, using plant pollens as the recipient, mixing the pollen and exogenous DNA in a 5%-50% sucrose solution subjected to aeration and low temperature treatment, transferring the exogenous gene into the recipient pollen under the assisted action of ultrasound, then pollinating the stigma of the plant with the treated pollen, and harvesting when the grains are mature; in the subsequent growing season, sowing the harvested seeds which are obtained after pollination of the transformed pollen, screening the germinated seeds and seedlings, carrying out a PCR amplification and Southern hybridization on the DNA of the samples of seedling plants, and further determining the transformants.
(FR)La présente invention concerne un procédé transgénique pour plante, qui comprend l'utilisation du plasmide d'Agrobacterium Ti, un plasmide d'Escherichia coli, ou d'autres vecteurs d'ADN comportant un fragment de gène exogène en tant que gène donneur, l'utilisation de pollens de plante en tant que receveur, le mélange du pollen et l'ADN exogène dans une solution de saccharose de 5 % à 50 % soumise à aération et une température de traitement basse, le transfert du gène exogène dans le pollen receveur sous l'action assistée d'ultrasons, puis la pollinisation du stigmate de la plante avec le pollen traité, et la récolte lorsque les grains sont matures ; dans la saison de croissance suivante, le semis des semences récoltées qui sont obtenues après la pollinisation du pollen transformé, le criblage des semences germées et des jeunes plantes, la conduite d'une amplification par PCR et d'une hybridation Southern sur l'ADN des échantillons de jeunes plantes, et ensuite la détermination des transformants.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)