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1. WO2012109788 - METHOD FOR INCREASING PROTEIN EXPRESSION EFFICIENCY AND EXPRESSION VECTOR THEREOF

Publication Number WO/2012/109788
Publication Date 23.08.2012
International Application No. PCT/CN2011/071030
International Filing Date 16.02.2011
IPC
C12N 15/63 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/67 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
CPC
C12N 15/67
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
Applicants
  • 中国水产科学研究院黄海水产研究所 YELLOW SEA FISHERIES RESEARCH INSTITUTE CHINESE ACADEMY OF FISHERY SCIENCES [CN]/[CN] (AllExceptUS)
  • 张庆利 ZHANG, Qingli [CN]/[CN] (UsOnly)
  • 黄倢 HUANG, Jie [CN]/[CN] (UsOnly)
  • 刘笋 LIU, Sun [CN]/[CN] (UsOnly)
  • 刘庆慧 LIU, Qinghui [CN]/[CN] (UsOnly)
  • 史成银 SHI, Chengyin [CN]/[CN] (UsOnly)
  • 梁艳 LIANG, Yan [CN]/[CN] (UsOnly)
Inventors
  • 张庆利 ZHANG, Qingli
  • 黄倢 HUANG, Jie
  • 刘笋 LIU, Sun
  • 刘庆慧 LIU, Qinghui
  • 史成银 SHI, Chengyin
  • 梁艳 LIANG, Yan
Agents
  • 青岛海昊知识产权事务所有限公司 QINGDAO HAIHAO INTELLECTUAL PROPERTY RIGHTS AGENT LTD.
Priority Data
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR INCREASING PROTEIN EXPRESSION EFFICIENCY AND EXPRESSION VECTOR THEREOF
(FR) PROCÉDÉ POUR AUGMENTER L'EFFICACITÉ D'EXPRESSION DE PROTÉINE ET VECTEUR D'EXPRESSION À CET EFFET
(ZH) 一种提高蛋白表达效率的方法及表达载体
Abstract
(EN) An expression vector for increasing protein expression efficiency is provided. A specific nucleotide sequence is introduced into the downstream of the initiation codon ATG of the expression vector, and encodes at least 3 amino acid residues. These amino acid residues process 4 or more codons. A corresponding method for increasing protein expression efficiency is also provided. The method of the present invention can be used in modification of any gene for increasing its expression efficiency. As compared to prior art, the expression vector or method of the present invention can increase the target protein expression efficiency by 2-5 times.
(FR) La présente invention concerne un vecteur d'expression pour augmenter l'efficacité d'expression de protéine. Une séquence nucléotidique spécifique est introduite en aval du codon d'initiation ATG du vecteur d'expression, et code pour au moins 3 résidus d'acide aminé. Ces résidus d'acide aminé correspondent à 4 codons ou plus. La présente invention concerne en outre un procédé pour augmenter l'efficacité d'expression de protéine. Le procédé de la présente invention peut être utilisé dans la modification d'un gène quelconque pour augmenter son efficacité d'expression. Par rapport à l'art antérieur, le vecteur d'expression ou procédé de la présente invention peuvent augmenter l'efficacité d'expression de protéine cible de 2 à 5 fois.
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