WO2012109788 METHOD FOR INCREASING PROTEIN EXPRESSION EFFICIENCY AND EXPRESSION VECTOR THEREOF

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Publication Number WO/2012/109788

Publication Date 23.08.2012

International Application No. PCT/CN2011/071030

International Filing Date 16.02.2011

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Applicants

中国水产科学研究院黄海水产研究所 YELLOW SEA FISHERIES RESEARCH INSTITUTE CHINESE ACADEMY OF FISHERY SCIENCES [CN]/[CN] (AllExceptUS)
张庆利 ZHANG, Qingli [CN]/[CN] (UsOnly)
黄倢 HUANG, Jie [CN]/[CN] (UsOnly)
刘笋 LIU, Sun [CN]/[CN] (UsOnly)
刘庆慧 LIU, Qinghui [CN]/[CN] (UsOnly)
史成银 SHI, Chengyin [CN]/[CN] (UsOnly)
梁艳 LIANG, Yan [CN]/[CN] (UsOnly)

Inventors

张庆利 ZHANG, Qingli
黄倢 HUANG, Jie
刘笋 LIU, Sun
刘庆慧 LIU, Qinghui
史成银 SHI, Chengyin
梁艳 LIANG, Yan

Agents

青岛海昊知识产权事务所有限公司 QINGDAO HAIHAO INTELLECTUAL PROPERTY RIGHTS AGENT LTD.

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Publication Language Chinese (zh)

Filing Language Chinese (ZH)

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Title

(EN) METHOD FOR INCREASING PROTEIN EXPRESSION EFFICIENCY AND EXPRESSION VECTOR THEREOF

(FR) PROCÉDÉ POUR AUGMENTER L'EFFICACITÉ D'EXPRESSION DE PROTÉINE ET VECTEUR D'EXPRESSION À CET EFFET

(ZH) 一种提高蛋白表达效率的方法及表达载体

Abstract

(EN) An expression vector for increasing protein expression efficiency is provided. A specific nucleotide sequence is introduced into the downstream of the initiation codon ATG of the expression vector, and encodes at least 3 amino acid residues. These amino acid residues process 4 or more codons. A corresponding method for increasing protein expression efficiency is also provided. The method of the present invention can be used in modification of any gene for increasing its expression efficiency. As compared to prior art, the expression vector or method of the present invention can increase the target protein expression efficiency by 2-5 times.

(FR) La présente invention concerne un vecteur d'expression pour augmenter l'efficacité d'expression de protéine. Une séquence nucléotidique spécifique est introduite en aval du codon d'initiation ATG du vecteur d'expression, et code pour au moins 3 résidus d'acide aminé. Ces résidus d'acide aminé correspondent à 4 codons ou plus. La présente invention concerne en outre un procédé pour augmenter l'efficacité d'expression de protéine. Le procédé de la présente invention peut être utilisé dans la modification d'un gène quelconque pour augmenter son efficacité d'expression. Par rapport à l'art antérieur, le vecteur d'expression ou procédé de la présente invention peuvent augmenter l'efficacité d'expression de protéine cible de 2 à 5 fois.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67	General methods for enhancing the expression

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67	General methods for enhancing the expression

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67	General methods for enhancing the expression

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1. WO2012109788 - METHOD FOR INCREASING PROTEIN EXPRESSION EFFICIENCY AND EXPRESSION VECTOR THEREOF