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Pub. No.:    WO/2012/106081    International Application No.:    PCT/US2012/021040
Publication Date: 09.08.2012 International Filing Date: 12.01.2012
C12Q 1/68 (2006.01), C12Q 1/48 (2006.01), G01N 33/53 (2006.01)
Applicants: ILLUMINA, INC. [US/US]; 5200 Illumina Way San Diego, California 92122 (US) (For All Designated States Except US).
ILLUMINA CAMBRIDGE LTD. [GB/GB]; Chesterford Research Park Nr Saffron Walden Essex CB10 1XL (GB) (For All Designated States Except US).
KLAUSING, Kay [DE/US]; (US) (For US Only).
SHEN, Min-Jui Richard [US/US]; (US) (For US Only).
MOORE, John [US/US]; (US) (For US Only).
SMITH, Vincent [GB/GB]; (GB) (For US Only).
HALL, Kevin [GB/GB]; (GB) (For US Only).
GORMLEY, Niall Anthony [GB/GB]; (GB) (For US Only).
IOANNOU, Avgousta [GB/GB]; (GB) (For US Only).
FRITZILAS, Epameinondas [GR/GB]; (GB) (For US Only).
RIGATTI, Roberto [IT/GB]; (GB) (For US Only)
Inventors: KLAUSING, Kay; (US).
SHEN, Min-Jui Richard; (US).
MOORE, John; (US).
SMITH, Vincent; (GB).
HALL, Kevin; (GB).
GORMLEY, Niall Anthony; (GB).
IOANNOU, Avgousta; (GB).
FRITZILAS, Epameinondas; (GB).
RIGATTI, Roberto; (GB)
Agent: THOMAS, Tiffany B.; 5200 Illumina Way San Diego, California 92122 (US)
Priority Data:
13/018,255 31.01.2011 US
61/438,522 01.02.2011 US
Abstract: front page image
(EN)Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample.
(FR)Cette invention concerne un procédé pour inhiber la dégradation des acides nucléiques pendant une étape de traitement des acides nucléiques choisie parmi la fragmentation et la détection, ledit procédé comprenant la mise en contact des acides nucléiques avec une solution comprenant de l'acide gallique, des analogues, dérivés ou mélanges de celui-ci, pendant l'étape de traitement, cette mise en contact inhibant la dégradation des acides nucléiques. Cette invention concerne également un procédé pour inhiber la dégradation induite par la lumière des acides nucléiques. De plus, cette invention concerne un procédé pour réduire ou inhiber les dommages occasionnés aux acides nucléiques pendant la préparation d'un échantillon d'acide nucléique comprenant la fragmentation des séquences d'acides nucléiques présentes dans l'échantillon dans une solution comprenant un ou plusieurs composés, lesdits composés inhibant la dégradation des séquences d'acides nucléiques dans l'échantillon.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)