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1. WO2012100963 - SEED TRAIT PREDICTION BY ACTIVITY-BASED PROTEIN PROFILING

Publication Number WO/2012/100963
Publication Date 02.08.2012
International Application No. PCT/EP2012/000396
International Filing Date 30.01.2012
Chapter 2 Demand Filed 28.11.2012
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 33/573
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
573for enzymes or isoenzymes
G01N 33/6842
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
G01N 33/6848
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
Applicants
  • MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V. [DE]/[DE] (AllExceptUS)
  • NINSAR AGROSCIENCES SL [ES]/[ES] (AllExceptUS)
  • GAUTIER SEMENCES [FR]/[FR] (AllExceptUS)
  • VAN DER HOORN, Renier, A., L. [DE]/[DE] (UsOnly)
  • GU, Chirstian [DE]/[DE] (UsOnly)
  • FARNUSCH, Kaschani [DE]/[DE] (UsOnly)
  • MISAS, Johana [DE]/[DE] (UsOnly)
  • RICHAU, Kerstin [DE]/[DE] (UsOnly)
  • KOLODZIEJEK, Izabella [DE]/[DE] (UsOnly)
  • MOQUET, Frédéric [FR]/[FR] (UsOnly)
  • MALLIART, Capucine [FR]/[FR] (UsOnly)
  • BERTHET, Xavier [ES]/[ES] (UsOnly)
  • RAMOS, Oscar, Goni [ES]/[ES] (UsOnly)
  • ELLUL, Philippe [ES]/[ES] (UsOnly)
Inventors
  • VAN DER HOORN, Renier, A., L.
  • GU, Chirstian
  • FARNUSCH, Kaschani
  • MISAS, Johana
  • RICHAU, Kerstin
  • KOLODZIEJEK, Izabella
  • MOQUET, Frédéric
  • MALLIART, Capucine
  • BERTHET, Xavier
  • RAMOS, Oscar, Goni
  • ELLUL, Philippe
Agents
  • MAIWALD PATENTANWALTS GMBH
Priority Data
11152509.328.01.2011EP
61/437,49628.01.2011US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SEED TRAIT PREDICTION BY ACTIVITY-BASED PROTEIN PROFILING
(FR) PRÉDICTION DES SPÉCIFICITÉS D'UNE GRAINE PAR PROFILAGE DES PROTÉINES EN FONCTION DE LEUR ACTIVITÉ
Abstract
(EN)
The present invention relates to a method of predicting a plant seed trait, such as germination rate, vigour, aging and priming, by determining the presence of a target protein, i.e. diagnostic marker, in its active state in a protein sample derived from a plant seed or plant seed lot. Thereby, the quality of a plant seed or a plant seed lot can be predicted and/or diagnosed and seeds may be distinguished on basis of their characteristics and with respect to the traits described herein. Inter alia, seeds and seed lots having high germination quality may be distinguished from seeds and seed lots having low germination quality. In particular, the method comprises contacting the protein sample or the plant seed or plant seed lot with a chemical probe comprising a warhead being able to attach to an amino acid residue in or nearby an active site of the target protein under conditions allowing the formation of a conjugate of the target protein and the chemical probe, wherein the chemical probe further comprises a reporter tag which is used to detect the conjugate, and wherein detection of the conjugate indicates the presence of the target protein in the protein sample and is used to predict or diagnose the plant seed trait. The present invention further relates to the use of such chemical probes for the prediction of plant seed traits.
(FR)
Cette invention concerne une méthode permettant de prédire une spécificité d'une graine végétale, tel que la vitesse de germination, la vigueur, le vieillissement et la réaction au traitement de prégermination, par détermination de la présence d'une protéine cible, à savoir, un marqueur diagnostique, à l'état actif dans un échantillon de protéines provenant d'une graine végétale ou d'un lot de graines végétales. De ce fait, la qualité d'une graine végétale ou d'un lot de graines végétales peut être prédite et/ou diagnostiquée et les graines peuvent être séparées en fonction de leurs caractéristiques et des spécificités ci-décrites. Inter alia, les graines et les lots de graines ayant une forte qualité de germination peuvent être séparés des graines et des lots de graines ayant une faible qualité de germination. En particulier, la méthode selon l'invention comprend la mise en contact de l'échantillon de protéines ou de la graine végétale ou du lot de graines végétales avec une sonde chimique comportant une ogive capable de se fixer à un résidu acide aminé dans ou à proximité du site actif de la protéine cible dans des conditions permettant la formation d'un conjugué constitué de la protéine cible et de la sonde chimique, la sonde chimique comprenant en outre une étiquette rapporteur qui sert à détecter le conjugué, la détection du conjugué indiquant la présence de la protéine cible dans l'échantillon de protéines et servant à prédire ou à diagnostiquer la spécificité d'une graine végétale. La présente invention concerne également l'utilisation desdites sondes chimiques pour prédire les spécificités d'une graine végétale.
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