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1. (WO2012099884) METHODS FOR AMPLIFICATION AND DETECTION OF PRIONS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/099884    International Application No.:    PCT/US2012/021561
Publication Date: 26.07.2012 International Filing Date: 17.01.2012
IPC:
G01N 33/68 (2006.01)
Applicants: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES [US/US]; National Institutes of Health Office of Technology Transfer 6011 Excutive Blvd., Suite 325 MSC 7660 Bethesda, MD 20892-7660 (US) (For All Designated States Except US).
PRIONICS AG [CH/CH]; Wagistrasse 27a CH-8952 Schlieren-Zurich (CH) (For All Designated States Except US).
ORRU, Christina, D. [IT/US]; (US) (For US Only).
CAUGHEY, Byron, W. [US/US]; (US) (For US Only).
KUHN, Franziska [CH/CH]; (CH) (For US Only).
SCHRÖDER, Björn [CH/CH]; (CH) (For US Only).
RAEBER, Alex [CH/CH]; (CH) (For US Only)
Inventors: ORRU, Christina, D.; (US).
CAUGHEY, Byron, W.; (US).
KUHN, Franziska; (CH).
SCHRÖDER, Björn; (CH).
RAEBER, Alex; (CH)
Agent: SIEGEL, Susan, Alpert; Klarquist Sparkman, LLP One World Trade Center, Suite 1600 121 SW Salmon Street Portland, OR 97204 (US)
Priority Data:
61/433,881 18.01.2011 US
Title (EN) METHODS FOR AMPLIFICATION AND DETECTION OF PRIONS
(FR) PROCÉDÉS D'AMPLIFICATION ET DE DÉTECTION DE PRIONS
Abstract: front page image
(EN)Methods are disclosed for detecting prions and/or prion disease-associated forms of prion protein. These methods provide sensitive and specific identification of prions in both biological and environmental samples. These methods include the use of both immunoprecipitation and an amplification assay that uses shaking in the absence of sonication, such as QuIC (SQ) or RT-QuIC (RTQ). In specific non- limiting examples, the methods include the use of monoclonal antibody 15B3 and/or RT-QuIC (RTQ), and/or a substrate replacement step.
(FR)L'invention concerne des procédés de détection de prions et/ou de formes associées à une maladie à prion d'une protéine prion. Ces procédés permettent une identification sensible et spécifique de prions à la fois dans des échantillons biologiques et environnementaux. Ces procédés comprennent l'utilisation à la fois de l'immunoprécipitation et d'un dosage par amplification qui utilise l'agitation en l'absence de sonification, telle que QuIC (SQ) ou RT-QuIC (RTQ). Dans des exemples spécifiques, qui ne sont pas limitants, les procédés comprennent l'utilisation d'un anticorps monoclonal 15B3 et/ou RT-QuIC (RTQ) et/ou une étape de remplacement de substrat.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)