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Machine translation
1. (WO2012091978) DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MULTIPOTENT NEURAL CREST CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/091978    International Application No.:    PCT/US2011/065810
Publication Date: 05.07.2012 International Filing Date: 19.12.2011
IPC:
C12N 5/079 (2010.01), C12N 5/0735 (2010.01), C12N 5/02 (2006.01)
Applicants: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. [US/US]; Boyd Graduate Studies Research Center D.W. Brooks Drive Athens, GA 30602-7411 (US) (For All Designated States Except US).
DALTON, Stephen [AU/US]; (US) (For US Only).
MENENDEZ, Laura, M. [US/US]; (US) (For US Only)
Inventors: DALTON, Stephen; (US).
MENENDEZ, Laura, M.; (US)
Agent: COLEMAN, Henry, D.; Coleman Sudol Sapone, P.C. 714 Colorado Avenue Bridgeport, CT 06605-1601 (US)
Priority Data:
61/429,344 03.01.2011 US
61/429,992 05.01.2011 US
61/548,045 17.10.2011 US
61/428,998 31.12.2010 US
Title (EN) DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MULTIPOTENT NEURAL CREST CELLS
(FR) DIFFÉRENCIATION DE CELLULES SOUCHES PLURIPOTENTES HUMAINES EN CELLULES DE CRÊTE NEURALE MULTIPOTENTE
Abstract: front page image
(EN)The present invention relates to the differentiation of human pluripotent cells, including human pluripotent stems cells to produce a self-renewing multipotent neural crest cell population in a single step method without the requirement of isolation of intermediate cells and without appreciable contamination (in certain preferred instances, virtually none) with Pax6+ neural progenitor cells in the population of p75+ Hnkl+ Ap2+ multipotent neural crest-like cells. The multipotent neural crest cell population obtained can be clonally amplified and maintained for >25 passages (>100 days) while retaining the capacity to differentiate into peripheral neurons, smooth muscle cells and mesenchymal precursor cells.
(FR)La présente invention concerne la différenciation de cellules pluripotentes humaines, comprenant des cellules souches pluripotentes humaines pour produire une population de cellules de crête neurale autorenouvelable dans un procédé à une étape sans nécessiter l'isolement de cellules intermédiaires et sans contamination appréciable (dans certains cas préférés, pratiquement aucune) avec des cellules progénitrices neurales Pax6+ dans la population de cellules de type crête neurale multipotentes p75+ Hnkl+ Ap2+. La population de cellules de crête neurale multipotentes obtenue peut être amplifiée de façon clonale et maintenue pendant > 25 passages (>100 jours) tout en conservant la capacité à se différencier en neurones périphériques, cellules de muscle lisse et cellules de précurseur de mésenchyme.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)