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1. WO2012085348 - METHOD OF PRODUCING HUMAN RETINAL PIGMENTED EPITHELIAL CELLS

Publication Number WO/2012/085348
Publication Date 28.06.2012
International Application No. PCT/FI2011/051142
International Filing Date 20.12.2011
IPC
C12N 5/071 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
C12N 5/0735 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
073Embryonic cells or tissues; Foetal cells or tissues
0735Embryonic stem cells; Embryonic germ cells
CPC
C12N 2501/59
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
50Cell markers; Cell surface determinants
59Lectins
C12N 2506/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2506Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
02from embryonic cells
C12N 5/0621
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0618Cells of the nervous system
0621Eye cells, e.g. cornea, iris pigmented cells
Applicants
  • GLYKOS FINLAND OY [FI]/[FI] (AllExceptUS)
  • SKOTTMAN, Heli [FI]/[FI] (UsOnly)
  • HONGISTO, Heidi [FI]/[FI] (UsOnly)
  • VAAJASAARI, Hanna [FI]/[FI] (UsOnly)
  • PARTANEN, Jukka [FI]/[FI] (UsOnly)
  • ALFTHAN, Kaija [FI]/[FI] (UsOnly)
Inventors
  • SKOTTMAN, Heli
  • HONGISTO, Heidi
  • VAAJASAARI, Hanna
  • PARTANEN, Jukka
  • ALFTHAN, Kaija
Agents
  • OY JALO ANT-WUORINEN AB
Priority Data
2010635420.12.2010FI
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF PRODUCING HUMAN RETINAL PIGMENTED EPITHELIAL CELLS
(FR) PROCÉDÉ DE PRODUCTION DE CELLULES ÉPITHÉLIALES PIGMENTÉES RÉTINIENNES HUMAINES
Abstract
(EN)
The present invention relates to the production of human retinal pigment epithelial cells comprising a step of incubating human pluripotent stem cells with binder molecules binding to terminal N-acetyllactosamine (Galβ1-4Glc NAc) and/or blood group H determinant type 2 (Fucα1-2Galβ1-4Glc NAc). Particularly, the invention relates to a use of lectin ECA in a culture of human pluripotent stem cells in order to support the stem cells to differentiate into retinal pigment epithelial cells.
(FR)
L'invention concerne la production de cellules épithéliales pigmentées rétiniennes humaines, consistant à incuber des cellules souches pluripotentes humaines avec des molécules de liaison se liant à la terminaison N-acétyllactosamine (Galβ1-4Glc NAc) et/ou au type 2 du déterminant H de groupe sanguin (Fucα1-2Galβ1-4Glc NAc). L'invention concerne en particulier l'utilisation de lectine ECA dans une culture de cellules souches pluripotentes humaines afin de favoriser la différenciation des cellules souches en cellules épithéliales pigmentées rétiniennes.
Also published as
Latest bibliographic data on file with the International Bureau