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1. (WO2012074386) TARGETED ALTERATION OF DNA WITH OLIGONUCLEOTIDES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2012/074386 International Application No.: PCT/NL2011/050805
Publication Date: 07.06.2012 International Filing Date: 25.11.2011
IPC:
C12N 15/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
KEYGENE N.V. [NL/NL]; P.O. Box 216 NL-6700 AE Wageningen, NL (AllExceptUS)
DE BOTH, Michiel Theodoor Jan [NL/NL]; NL (UsOnly)
FURUKAWA, Tomoyuki [JP/NL]; NL (UsOnly)
Inventors:
DE BOTH, Michiel Theodoor Jan; NL
FURUKAWA, Tomoyuki; NL
Agent:
RAGGERS, R.J.; Exter Polak & Charlouis B.V. P.O. Box 3241 NL-2280 GE RIJSWIJK, NL
Priority Data:
200580903.12.2010NL
61/419,18302.12.2010US
Title (EN) TARGETED ALTERATION OF DNA WITH OLIGONUCLEOTIDES
(FR) ALTÉRATION CIBLÉE D'ADN AVEC DES OLIGONUCLÉOTIDES
Abstract:
(EN) The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises use of at least two oligonucleotides, each oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch of the first oligonucleotide is directed to a nucleotide at a position in the first strand of the duplex and the mismatch of the second oligonucleotide is directed to the nucleotide in the second strand that occupies the complementary position in the duplex acceptor DNA (e.g. forms a base-pair with the nucleotide in the first strand). These mismatches are located at specific positions within said oligonucleotides. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises oligonucleotides suitable for use in the method.
(FR) La présente invention concerne un procédé pour l'altération ciblée d'ADN accepteur, par exemple d'ADN duplex accepteur. Le procédé comprend l'utilisation d'au moins deux oligonucléotides, chaque oligonucléotide ayant au moins un mauvais appariement par rapport à l'ADN accepteur ciblé (duplex). Le mauvais appariement du premier oligonucléotide est dirigé vers un nucléotide à une position dans le premier brin du duplex et le mauvais appariement du second oligonucléotide est dirigé vers le nucléotide dans le second brin qui occupe la position complémentaire dans l'ADN duplex accepteur (par exemple, forme une paire de bases avec le nucléotide dans le premier brin). Ces mauvais appariements sont localisés à des positions spécifiques dans lesdits oligonucléotides. L'invention concerne aussi une trousse qui comprend des instructions pour exécuter le procédé selon l'invention, et dans une forme de réalisation préférée, comprend des oligonucléotides appropriés pour l'utilisation dans le procédé.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
CA2819431US20130330774JP2014502161EP2646550CN103502449ES2536640
AU2011337324DK2646550US20170121706IN4196/CHENP/2013