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1. WO2012068446 - VIABILITY CELL COUNTING BY DIFFERENTIAL LIGHT ABSORPTION

Publication Number WO/2012/068446
Publication Date 24.05.2012
International Application No. PCT/US2011/061363
International Filing Date 18.11.2011
IPC
C12Q 1/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
G01N 33/48 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
CPC
C12Q 1/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
G01N 2015/1486
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1486Counting the particles
Applicants
  • BIO-RAD LABORATORIES, INC. [US]/[US] (AllExceptUS)
  • HENG, Xin [CN]/[US] (UsOnly)
  • BRENIMAN, Eugene [US]/[US] (UsOnly)
Inventors
  • HENG, Xin
  • BRENIMAN, Eugene
Agents
  • HEINES, M. Henry
Priority Data
61/414,94718.11.2010US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) VIABILITY CELL COUNTING BY DIFFERENTIAL LIGHT ABSORPTION
(FR) COMPTAGE CELLULAIRE DE VIABILITÉ PAR ABSORPTION LUMINEUSE DIFFÉRENTIELLE
Abstract
(EN)
Cell counts that distinguish between live and dead cells while providing an accurate count of the total of live and dead cells are obtained by the use of a vital stain in conjunction with illumination of the cell population and the taking of light images at different wavelengths, one which is not absorbed by the stain and one that is absorbed by the stain. Masking and inaccuracies in the counting of dead cells is thereby avoided.
(FR)
La présente invention concerne des comptes cellulaires permettant de distinguer des cellules vives et mortes tout en fournissant un compte précis du nombre total des cellules vives et mortes. Lesdits comptes cellulaires sont obtenus en utilisant une tache vitale conjointement avec l'éclairage de la population cellulaire et la prise d'images lumineuses à des longueurs d'onde différentes, dont une n'est pas absorbée par la tache et dont une est absorbée par la tache. Le masquage et des imprécisions du comptage de cellules mortes sont ainsi évités.
Also published as
Latest bibliographic data on file with the International Bureau